Strain Detail Sheet

Strain Name    :

STOCK Plxna4tm1Matl/Mmmh

Stock Number :

030407-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Plxna4tm1Matl
Name: targeted mutation 1, Marc Tessier-Lavigne
Alteration at locus: Knockout
Gene Symbol: Plxna4
Name: plexin A4
Chromosome: 6
Alteration at locus: Knockout

Genetic Alterations:
ER retention signal & Neomycin cassette inserted into exon 18 & 19

Genotype Determination:

ES Cell Line: E14 derived from STOCK

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Defects in axonal branching

Heterozygous phenotype: None


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Plxna4tm1Matl/Plxna4tm1Matl
        either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * CD-1)
  • nervous system phenotype
    • abnormal axon guidance (MGI Ref ID J:98440)
      • repulsive effect of Sema3A on axon growth from sensory neurons from the dorsal root ganglia was reduced compared to wildtype, with 10-20% of axons not repelled at all while the remaining axons were repelled but they fanned out broadly instead of being tightly fasciculated as in wildtype
      • repulsive response of superior cervical ganglion neurons to Sema3A, but not Sema3F, was partially reduced
    • abnormal sensory neuron projections (MGI Ref ID J:98440)
      • the spinal sensory axons failed to form thick nerve bundles at E11.5 and showed enhanced branching at E12.5 in the limb buds
      • observed a misprojecting fiber bundle along the ventral part of the paw
    • abnormal trigeminal nerve morphology (MGI Ref ID J:98440)
      • disorganized ophthalmic and maxillary projections with the ophthalmic branch overshooting beyond the normal area and some fibers leaving the main bundle to grow in different directions at E11.5, however all three projections of the trigeminal ganglion generally projected to their correct targets
    • decreased anterior commissure size (MGI Ref ID J:98440)
      • anterior commissure tract was reduced in size and defasciculated
Plxna4tm1Matl/Plxna4tm1Matl
        involves: 129P2/OlaHsd
  • nervous system phenotype
    • abnormal amacrine cell morphology (MGI Ref ID J:172268)
      • dopaminergic amacrine cells extend aberrant processes into the S4/S5 sublamina of the inner plexiform layer unlike in wild-type mice
    • abnormal neurite morphology (MGI Ref ID J:172268)
      • dopaminergic amacrine cells extend aberrant processes into the S4/S5 sublamina of the inner plexiform layer unlike in wild-type mice
      • calbindin+ cells extend processes into the S4/S5 sublamina of the IPL of the retina and exhibit abnormal arborization unlike in wild-type mice
      • M1-type intrinsically photosensitive retinal ganglion cells (ipRGCs) exhibit aberrant dendritic arborization in the S4/S5 sublamina of the inner plexiform layer (IPL) and stratification within S1 of the IPL unlike in wild-type mice
      • aberrant dopaminergic amacrine cell processes co-localize with M1-type ipRGC dendrites
      • however, other subtypes of retinal ganglion and amacrine cells exhibit normal neurite arborization and neurite fasciculation of retinal cells types is normal
    • abnormal retinal ganglion cell morphology (MGI Ref ID J:172268)
      • M1-type intrinsically photosensitive retinal ganglion cells (ipRGCs) exhibit aberrant dendritic arborization in the S4/S5 sublamina of the inner plexiform layer (IPL) and stratification within S1 of the IPL unlike in wild-type mice
      • aberrant dopaminergic amacrine cell processes co-localize with M1-type ipRGC dendrites
      • however, other subtypes of retinal ganglion and amacrine cells exhibit normal neurite arborization
  • vision/eye phenotype
    • abnormal amacrine cell morphology (MGI Ref ID J:172268)
      • dopaminergic amacrine cells extend aberrant processes into the S4/S5 sublamina of the inner plexiform layer unlike in wild-type mice
    • abnormal retinal ganglion cell morphology (MGI Ref ID J:172268)
      • M1-type intrinsically photosensitive retinal ganglion cells (ipRGCs) exhibit aberrant dendritic arborization in the S4/S5 sublamina of the inner plexiform layer (IPL) and stratification within S1 of the IPL unlike in wild-type mice
      • aberrant dopaminergic amacrine cell processes co-localize with M1-type ipRGC dendrites
      • however, other subtypes of retinal ganglion and amacrine cells exhibit normal neurite arborization

Strain of Origin: C57BL/6

Strain genetic background: Mixed C57BL/6 & CD1

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Marc Tessier-Lavigne, Ph.D., Genentech

Primary Reference:

Yaron A, Huang PH, Cheng HJ, Tessier-Lavigne M. Differential requirement for Plexin-A3 and -A4 in mediating responses of sensory and sympathetic neurons to distinct class 3 Semaphorins. Neuron. 2005 Feb 17;45(4):513-23. (Medline PMID: 15721238)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030407-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030407-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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