Strain Detail Sheet

Strain Name    :

B6;129P2-Tg(NKX2-5-EmGFP)2Conk/Mmnc

Stock Number :

030440-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Tg(NKX2-5-EmGFP)2Conk
Name: transgene insertion 2, Bruce Conklin
Alteration at locus: Transgenic (BAC)
Transgene: NKX2-5 (Human)
Name: NK2 transcription factor related, locus 5
Alteration at locus: Transgenic (BAC)
Reporter: EmGFP (Jelly Fish)
Name: Emerald Green Fluorescent Protein
Alteration at locus: Transgenic (BAC)

Genetic Alterations:
Mouse E14 ES cells were electroporated with a modified human BAC for NKX2-5-EmGFP to make the transgenic reporter line. The BAC is based on RP11-88L12, and carries an in-frame P2A-EmGFP insertion, a Zeocin cassette, and a SV40- Neo selection cassette.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Early cardiac progenitors are tagged with Emerald GFP. Embryonic heart tissues are readily identified by green fluorescence. In addition, other tissues expressing NKX2-5 (eg foregut, branchial arches) will be labeled by GFP.

Heterozygous phenotype:


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Tg(NKX2-5-EmGFP)2Conk/-
        involves: 129P2/OlaHsd
  • no phenotypic analysis
    • no phenotypic analysis (MGI Ref ID J:160582)

Strain of Origin: 129P2/OlaHsd

Strain genetic background: Mixed 129OLA/C57BL/6

Strain Development: Mouse was created by blastocyst injection of E14 ES cells carrying a modified human BAC transgene for NKX2-5-EmGFP (the BAC is based on RP11-88L12, and carries an in-frame P2A-EmGFP insertion and Zeocin and SV40-Neo selection cassettes). A single chimera showed germline transmission, and this founder was used to start the colony. The mouse has been backcrossed once to the C57BL/6 background.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6;129P2-Tg(NKX2-5-EmGFP)2Conk MMRRC:030440-UNC C57BL/6J 129P2/OlaHsd 2173 0 409 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Internal/Organ
  • Research Tools

Strain Origin

Donor: Bruce Conklin, M.D., J. David Gladstone Institutes

Primary Reference:

  • van Laake LW, Qian L, Cheng P, Huang Y, Hsiao EC, Conklin BR, Srivastava D. Reporter-based isolation of induced pluripotent stem cell- and embryonic stem cell-derived cardiac progenitors reveals limited gene expression variance. Circ Res. 2010 Aug 6;107(3):340-7. Epub 2010 Jun 17. (Medline PMID: 20558827)
  • Hsiao EC, Yoshinaga Y, Nguyen TD, Musone SL, Kim JE, Swinton P, Espineda I, Manalac C, deJong PJ, Conklin BR. Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter. PLoS ONE. 2008 Jul 2;3(7):e2532. (Medline PMID: 18596956 )

Colony and Husbandry Information

Special Considerations

See also: MMRRC:030473

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030440-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/24/2014