Strain Name:
B6.129S1(Cg)-Slc6a1tm1.1Lst/Mmucd
Stock Number:
030468-UCD
Citation ID:
RRID:MMRRC_030468-UCD
Other Names:
D2Cre, C57BL/6TgHGAT1GFP(GA12), B6.129S1(Cg)-Slc6a1tm1.1Lst/Mmcd

Strain Information

Slc6a1tm1.1Lst
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1; targeted mutation 1.1, Henry A Lester
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
Slc6a1
Name: solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Synonyms: XT-1, Xtrp1, Gabt1, Gat1, Gabt, GAT-1
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knock-In
NCBI: 232333
Homologene: 2290
Genetic Alterations
Donor isolated 26 genomic clones and identified one clone that carried intron 14 and exon 15 and had >4.0 kb of flanking genomic sequence on both arms.The floxed Neo cassette was modified by creating AscI sites on both ends by linkers. An additional 1.6 kb flanking arm at the 3' end of this construct was created by replacing the ApaI/EcoRI sites in the pKO vector with the corresponding mGAT1 genomic DNA sites. The final construct has ~3.8 kb on the 5' arm, -Neo-320 bp-GFP-, and ~4.2 kb on the 3' arm for homologous recombination. The 320 bp sequence includes the 34 residues of the coding portion of exon 15. The ES cell clones that survived this screening were further screened by PCR (Long template PCR kit; Roche) using a primer set that anneals to position 13017-43 and the neomycin resistance cassette (Neo): Positive clones were confirmed by PCR using additional sets of primers.Two clones were injected into the C57 blastocyst. Chimeras that displayed >60% agouti coloring were mated with C57 females to generate heterozygous mice. These mice carry the Neo- cassette in intron 14 and were named intron 14-Neo-mGAT1. The loxP- flanked Neo-cassette was deleted by mating with DBA mice carrying cre recombinase. This deletion leaves the 34 bp of one loxP site plus restriction sites (SwaI-AscI) in the intron, and this Neo-deleted strain was named mGAT1-GFP.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: The patterns of flourescence and immunohistochemical finding strongly suggests that GAT1 is expressed in axons, in synapses and in astrocytes in many brain regions.The mGAT1-GFP mouse displays apparently normal life span, reproductive behavior, and weight, and mostly normal tonic and phasic inhibitory synaptic transmission.

Heterozygous: Homozygotes and heterozygotes from the mGAT1-GFP line display normal weight, development, and life span, and, on the basis of anecdotal observations, normal behavior.
Mammalian Phenotype Terms
Allelic Composition: Slc6a1tm1.1Lst/Slc6a1tm1.1Lst (Genetic Background: involves: 129S1/Sv )

MeSH Terms
  • Animals
  • Biotinylation
  • Carrier Proteins/biosynthesis
  • Carrier Proteins/genetics
  • Cell Line
  • Cell Membrane/metabolism
  • Cerebellum/cytology
  • Cerebellum/metabolism
  • Cytoplasm/metabolism
  • DNA, Complementary/genetics
  • DNA, Complementary/metabolism
  • GABA Plasma Membrane Transport Proteins
  • Green Fluorescent Proteins
  • Hippocampus/cytology
  • Hippocampus/metabolism
  • Humans
  • Kidney/cytology
  • Kidney/metabolism
  • Luminescent Proteins/genetics
  • Membrane Proteins/biosynthesis
  • Membrane Proteins/genetics
  • Membrane Transport Proteins
  • Mice
  • Mice, Transgenic
  • Microscopy, Fluorescence
  • Neurons/cytology
  • Neurons/metabolism
  • Organ Specificity
  • Organic Anion Transporters
  • Patch-Clamp Techniques
  • Presynaptic Terminals/metabolism
  • Recombinant Fusion Proteins/biosynthesis
  • Recombinant Fusion Proteins/genetics
  • Stem Cells/cytology
  • Synaptic Transmission/physiology
  • gamma-Aminobutyric Acid/metabolism
Strain Development
GAT1-deficient mice were generated as an intermediate in the construction of the mGAT1-GFP strain (Chiu et al 2002) By homologous recombination, a 2.2.Kb neo cassette was inserted into ontron14 of the GAT1-GFP fusion gene in ES cells. Blastocysts were injected with recombinant ES cells and implanted into C57BL/6 females ( Chiu et al 2002)
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools
Donor
Henry A Lester, Ph.D., California Institute of Technology
Primary Reference
Chiu CS, Jensen K, Sokolova I, Wang D, Li M, Deshpande P, Davidson N, Mody I, Quick MW, Quake SR, Lester HA. Number, density, and surface/cytoplasmic distribution of GABA transporters at presynaptic structures of knock-in mice carrying GABA transporter subtype 1-green fluorescent protein fusions. J Neurosci. 2002 Dec 1;22(23):10251-66. (Medline PMID: 12451126)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030468-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030468-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
030468-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.