Strain Name:
STOCK Sox8tm1Mkob/Mmnc
Stock Number:
030471-UNC
Citation ID:
RRID:MMRRC_030471-UNC

Strain Information

Sox8tm1Mkob
Name: SRY (sex determining region Y)-box 8; targeted mutation 1, Moira K O'Bryan
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knockout
Sox8
Name: SRY (sex determining region Y)-box 8
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Knockout
NCBI: 20681
Homologene: 7950
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2
    Phenotype
    Homozygous: Progressive male infertility

    Heterozygous: Not characterized.
    Mammalian Phenotype Terms
    Allelic Composition: Sox8tm1Mkob/Sox8tm1Mkob (Genetic Background: involves: 129S7/SvEvBrd )

    MeSH Terms
    • Animals
    • DNA-Binding Proteins/deficiency
    • DNA-Binding Proteins/genetics
    • DNA-Binding Proteins/physiology
    • Exons
    • Fertility/physiology
    • Gene Deletion
    • Genotype
    • Male
    • Mice/embryology
    • Mice/genetics
    • Mice, Knockout
    • Mice, Transgenic
    • Polymerase Chain Reaction
    • Restriction Mapping
    • SOXE Transcription Factors
    • Sertoli Cells/physiology
    • Testis/cytology
    • Testis/embryology
    • Testis/physiology
    • Transcription Factors/deficiency
    • Transcription Factors/genetics
    • Transcription Factors/physiology
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

    GQC analysis was done on two XY samples LY0870, QK5899 at the time of importation and the conclusions are consistent in both samples. The results of the analysis are congruent with the SDS, although the strain description is incomplete. QK5899 is the representative sample selected for the report. A 129S7SvEv reference is not available in miniMUGA and is the donor ES cell background indicated in the SDS, therefore, the haplotype reconstruction at the locus is unreliable. The primary background is consistent with the 129S strain group, there is positive evidence of 129S6 and 129S2 diagnostics. The secondary background is consistent with C57BL6/N, which is consistent with the nomenclature and the SDS. The mitochondrial and Y genomes are consistent with a strain maintained in a mixed 129S and C57BL6/N backgrounds. None of the constructs detectable by MiniMUGA are present.

    The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible.
    MiniMUGA Sample Report
    • Cardiovascular
    • Developmental Biology
    • Models for Human Disease
    • Neurobiology
    • Reproduction
    • Bone Biology
    Donor
    Yuji Mishina, Ph.D., National Institutes of Health, National Institute of Environmental Health Science
    Primary Reference
    O'Bryan MK, Takada S, Kennedy CL, Scott G, Harada S, Ray MK, Dai Q, Wilhelm D, de Kretser DM, Eddy EM, Koopman P, Mishina Y. Sox8 is a critical regulator of adult Sertoli cell function and male fertility. Dev Biol. 2008 Apr 15;316(2):359-70. Epub 2008 Feb 13. (Medline PMID: 18342849)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    030471-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    030471-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    030471-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.