Strain Detail Sheet

Strain Name    :

B6;129S7-Sox8tm1Mkob/Mmnc

Stock Number :

030471-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Sox8tm1Mkob
Name: targeted mutation 1, Moira K O'Bryan
Alteration at locus: Knockout

Genetic Alterations:

Genotype Determination:

ES Cell Line: AB2.2 derived from 129S7/SvEvBrd

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Progressive male infertility

Heterozygous phenotype: Unknown


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Sox8tm1Mkob/Sox8tm1Mkob
        involves: 129S7/SvEvBrd
  • growth/size phenotype
    • slow postnatal weight gain (MGI Ref ID J:136098)
      • mice have significantly lower weight than controls at 2 months of age
  • reproductive system phenotype
    • abnormal fertility/fecundity (MGI Ref ID J:136098)
      • decreased litter size (MGI Ref ID J:136098)
        • litters sired by mutant males are of smaller size and litter sizes decrease with age of the mutant father
      • reduced male fertility (MGI Ref ID J:136098)
        • there is reduced fertility among male mice that gets worse with age
        • 3 of 8 young males are sterile, 2 produce only one litter, 1 produces two litters and 2 produce 4 litters
        • no males are capable of siring litters after 150 days of age
    • abnormal male reproductive system morphology (MGI Ref ID J:136098)
      • abnormal Sertoli cell morphology (MGI Ref ID J:136098)
        • cytoplasm of Sertoli cells in 5 month old males are vacuolated and have a lacy appearance
      • abnormal epididymis morphology (MGI Ref ID J:136098)
        • 5 month old males have a massive increase in the number of round spermatid found in the lumen of the epididymis
        • spermatid and spermatocytes are visible within the epididymidal lumen by 1 month of age and maintained until at least 9 months of age
      • abnormal seminiferous epithelium morphology (MGI Ref ID J:136098)
        • increase vacuolation occurs in the seminiferous epithelium of 5 month old males that is suggestive of germ cell loss
      • decreased testis weight (MGI Ref ID J:136098)
        • testis have lower weight both in absolute and relative to body weight measurements compared to control males starting at 2 months of age
        • testis weight is 82% of controls at 2 months of age and 59% by 9 months of age
    • abnormal sperm number (MGI Ref ID J:136098)
      • caudal epididymal sperm counts are reduced and worsen with age
    • abnormal spermatogenesis (MGI Ref ID J:136098)
      • male germ cells are often found at inappropriate depth in the seminiferous epithelium or adjacent to germ cells at different stages of spermatogenesis
      • examples of this include round spermatid in stage X tubules or diplotene spermatocytes juxtapositioned with round spermatids within the same tubule
      • this disorganization increases with age of the male
      • abnormal spermatid morphology (MGI Ref ID J:136098)
        • decrease number of elongating/elongated spermatids occurs in the testis of 5 month old males
        • total number of elongated spermatid in 9 month old testis is significantly reduced but some are still present
      • abnormal spermiation (MGI Ref ID J:136098)
        • increased numbers of elongated spermatid are retained in the seminiferous epithelium of 5 month old males
        • stage VIII and stage IX spermatid are often not orientated in the proper direction with acrosomes pointed away from the basement membrane
        • spermiation failure becomes progressively more abundant with age
        • failure of apical ectoplasmic specialization resolution between spermatids and Sertoli cells is observed at high resolution
    • asthenozoospermia (MGI Ref ID J:136098)
      • the percentage of sperm with motility is half that of controls at 2 months of age whether progressive or total motility is measured
      • sperm motility is almost completely absent by 5 months of age
  • endocrine/exocrine gland phenotype
    • abnormal Sertoli cell morphology (MGI Ref ID J:136098)
      • cytoplasm of Sertoli cells in 5 month old males are vacuolated and have a lacy appearance
    • abnormal seminiferous epithelium morphology (MGI Ref ID J:136098)
      • increase vacuolation occurs in the seminiferous epithelium of 5 month old males that is suggestive of germ cell loss
    • decreased testis weight (MGI Ref ID J:136098)
      • testis have lower weight both in absolute and relative to body weight measurements compared to control males starting at 2 months of age
      • testis weight is 82% of controls at 2 months of age and 59% by 9 months of age

Strain of Origin: C57BL6/129SvEv

Strain genetic background: C57BL6/129SvEv

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Bone Biology

Strain Origin

Donor: Yuji Mishina, Ph.D., NIH, National Institute of Environmental Health Science

Primary Reference:

O'Bryan MK, Takada S, Kennedy CL, Scott G, Harada S, Ray MK, Dai Q, Wilhelm D, de Kretser DM, Eddy EM, Koopman P, Mishina Y. Sox8 is a critical regulator of adult Sertoli cell function and male fertility. Dev Biol. 2008 Apr 15;316(2):359-70. Epub 2008 Feb 13. (Medline PMID: 18342849)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030471-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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