Strain Name:
C.129P2(B6)-Mcpt1tm1Hrpm/Mmmh
Stock Number:
030488-MU
Citation ID:
RRID:MMRRC_030488-MU

Strain Information

Mcpt1tm1Hrpm
Name: mast cell protease 1; targeted mutation 1, Hugh R P Miller
Synonyms: mMCP-1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
Mcpt1
Name: mast cell protease 1
Synonyms: Mcp-1
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Knockout
NCBI: 17224
VEGA: 14
Homologene: 137209
Genetic Alterations
All 5 exons replaced by neo gene
Genotype Determination
ES Cell Line
E14TG2a derived from 129P2/OlaHsd
Phenotype
Homozygous: Reduced mucosal mast cell activity. Otherwise normal.

Heterozygous: Normal responses
Mammalian Phenotype Terms
Allelic Composition: Mcpt1tm1Hrpm/Mcpt1tm1Hrpm (Genetic Background: involves: 129P2/OlaHsd * MF1 )

MeSH Terms
  • Animals
  • Cell Count
  • Chymases
  • Connective Tissue/metabolism
  • Enzyme-Linked Immunosorbent Assay
  • Esterases/metabolism
  • Immunity, Mucosal
  • Immunoenzyme Techniques
  • Inclusion Bodies/immunology
  • Intestinal Mucosa/enzymology
  • Intestinal Mucosa/immunology
  • Intestinal Mucosa/ultrastructure
  • Mast Cells/cytology
  • Mast Cells/enzymology
  • Mast Cells/ultrastructure
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Electron
  • Nippostrongylus
  • Polymerase Chain Reaction
  • Serine Endopeptidases/deficiency
  • Serine Endopeptidases/genetics
  • Serine Endopeptidases/metabolism
  • Serine Endopeptidases/physiology
  • Strongylida Infections/immunology
  • Gene Deletion
  • Histocytochemistry
  • Intestinal Mucosa/parasitology
  • Jejunum/immunology
  • Jejunum/parasitology
  • Mast Cells/immunology
  • Mice, Congenic
  • Mice, Inbred BALB C
  • Muscles/parasitology
  • RNA, Messenger/analysis
  • RNA, Messenger/genetics
  • Trichinella spiralis/immunology
  • Trichinella spiralis/physiology
  • Trichinellosis/immunology
  • Trichinellosis/parasitology
Strain Development
Using 129 ES cell mixed with C57BL/6 (from Harlan labs in the UK) blastocysts. Founders backcrossed to MF-1 strain and then for 10 generations to BALB/c strain (Harlan UK) and then twice to BALB/cAnTac in the US.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Immunology and Inflammation
  • Models for Human Disease
Donor
Michael F. Gurish, Ph.D., Brigham and Women's Hospital
Primary Reference
  • Wastling JM, Knight P, Ure J, Wright S, Thornton EM, Scudamore CL, Mason J, Smith A, Miller HR. Histochemical and ultrastructural modification of mucosal mast cell granules in parasitized mice lacking the beta-chymase, mouse mast cell protease-1. Am J Pathol. 1998 Aug;153(2):491-504. (Medline PMID: 9708809)
  • Knight PA, Wright SH, Lawrence CE, Paterson YY, Miller HR. Delayed expulsion of the nematode Trichinella spiralis in mice lacking the mucosal mast cell-specific granule chymase, mouse mast cell protease-1. J Exp Med. 2000 Dec 18;192(12):1849-56. (Medline PMID: 11120781)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030488-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
030488-MU-RESUS Litter recovered from cryo-archive $2,697.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.