Strain Name    : B6;129-Ireb2tm1Roua/Mmmh

Stock Number : 030490-MU

Gene Information

(provided by MGI)
Allele Symbol: Ireb2tm1Roua
Name: targeted mutation 1, Tracey Rouault
Chromosome: 9
Alteration at locus: Knockout
Gene Symbol: Ireb2
Name: iron responsive element binding protein 2
Chromosome: 9
Alteration at locus: Knockout
Genetic Alterations:
Embryonic stem cells were transfected with an Ireb2-mutant construct made using a genomic Ireb2 clone derived from a 129/Sv library and the targeting vector pPNT. Donor subcloned a 1.8-kb SstI (blunted)-BglII fragment of the Ireb2 clone, containing parts of exons 2 and 3, into the EcoRI (blunted)-BamHI sites of pPNT, generating the 5

Genotype Determination:

ES Cell Line:

Phenotype

Homozygous phenotype: progressive neurodegenerative disorder - cerebellar ataxia, axonopathy iron limited erythropoiesis, anemia, erythropoietic protoporphyria.

Heterozygous phenotype: unaffected.

Mammalian Phenotype Terms:
Allelic Composition: Ireb2tm1Roua/Ireb2tm1Roua (Genetic Background: B6.Cg-Ireb2tm1Roua )

Allelic Composition: Ireb2tm1Roua/Ireb2tm1Roua (Genetic Background: involves: C57BL/6 )

Strain of Origin: B6;129S4

Strain genetic background: B6;129S4

Suggested Control Mice:

  • Wildtype littermates

  • Hematology
  • Metabolism
  • Models for Human Disease
  • Neurobiology

Donor: Tracey A. Rouault, M.D., NICHD

Primary Reference:

LaVaute T, Smith S, Cooperman S, Iwai K, Land W, Meyron-Holtz E, Drake SK, Miller G, Abu-Asab M, Tsokos M, Switzer R 3rd, Grinberg A, Love P, Tresser N, Rouault TA. Targeted deletion of the gene encoding iron regulatory protein-2 causes misregulation of iron metabolism and neurodegenerative disease in mice. Nat Genet. 2001 Feb;27(2):209-14. (Medline PMID:11175792 )

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
030490-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030490-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.