Strain Name    : C57BL/6J-Dock7mnlt/Mmucd

Stock Number : 030498-UCD

Other Names   : moonlight, C57BL/6J-Dock7mnlt/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Dock7mnlt
Name: moonlight
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Dock7
Name: dedicator of cytokinesis 7
Chromosome: 4
Alteration at locus: Chemically Induced
Genetic Alterations:
The moonlight mutation was mapped to Chromosome 4. Sequencing of the critical region found a deletion spanning exons 22-28 (of 48 total exons) of the Dock7 gene (positions 118824 to137848 occurring in the middle of introns 22 and 29 in the Genbank genomic region NC_000070 for linear genomic DNA sequence of Dock7). If the donor and acceptor splice sites of introns 22 and 29 are still used, amino acids 866-1173 of the DOCK7 protein would be deleted, and a frameshift and insertion of 30 aberrant amino acids before a premature stop codon would occur.

Genotype Determination:

    ES Cell Line: Not applicable

    Additional mutations found on this strain:

    AlleleSourceChrCoordTypePredicted EffectPPH Score
    IGL00159:Dock7 APN 4 99063985 missense possibly damaging 0.80
    IGL01126:Dock7 APN 4 98973552 splice acceptor site 0.00
    IGL01490:Dock7 APN 4 98945118 splice donor site 0.00
    IGL01553:Dock7 APN 4 98945566 nonsense probably null 0.00
    IGL01728:Dock7 APN 4 98962331 missense probably damaging 1.00
    IGL01776:Dock7 APN 4 98940941 missense possibly damaging 0.45
    IGL01954:Dock7 APN 4 99083151 missense possibly damaging 0.93
    IGL01985:Dock7 APN 4 99023377 missense probably benign 0.02
    IGL02054:Dock7 APN 4 98973409 missense possibly damaging 0.44
    IGL02150:Dock7 APN 4 99079852 splice site 0.00
    IGL02153:Dock7 APN 4 98958067 missense probably benign 0.00
    IGL02183:Dock7 APN 4 98958991 missense probably benign 0.01
    IGL02494:Dock7 APN 4 98989234 missense probably benign 0.25
    IGL02618:Dock7 APN 4 99083028 missense probably benign 0.00
    IGL02634:Dock7 APN 4 98989296 missense probably damaging 1.00
    IGL02670:Dock7 APN 4 98966286 splice site 0.00
    IGL02690:Dock7 APN 4 98969635 missense possibly damaging 0.52
    IGL02692:Dock7 APN 4 98987386 missense probably benign 0.22
    R0086:Dock7 UTSW 4 98945144 missense probably damaging 1.00
    R0242:Dock7 UTSW 4 98962280 missense probably benign 0.00
    R0242:Dock7 UTSW 4 98962280 missense probably benign 0.00
    R0245:Dock7 UTSW 4 99055349 missense probably benign 0.29
    R0308:Dock7 UTSW 4 98984814 missense probably benign 0.01
    R0556:Dock7 UTSW 4 98945189 missense probably damaging 1.00
    R0612:Dock7 UTSW 4 98989233 missense probably benign 0.00
    R0652:Dock7 UTSW 4 99055349 missense probably benign 0.29
    R0669:Dock7 UTSW 4 98987479 missense probably benign 0.00
    R0681:Dock7 UTSW 4 99016704 missense probably damaging 1.00
    R0725:Dock7 UTSW 4 98945291 missense possibly damaging 0.95
    R0828:Dock7 UTSW 4 99015745 missense probably damaging 1.00
    R0837:Dock7 UTSW 4 98989258 missense probably benign 0.01
    R0962:Dock7 UTSW 4 98945195 missense possibly damaging 0.85
    R1140:Dock7 UTSW 4 99065406 missense possibly damaging 0.82
    R1476:Dock7 UTSW 4 99079435 missense probably benign 0.01
    R1614:Dock7 UTSW 4 99061280 missense probably benign 0.03
    R1625:Dock7 UTSW 4 98962196 splice donor site probably null
    R1640:Dock7 UTSW 4 98945246 missense probably damaging 1.00
    R1752:Dock7 UTSW 4 98966444 missense probably damaging 1.00
    R1941:Dock7 UTSW 4 98984715 missense probably benign 0.09
    R2010:Dock7 UTSW 4 98970904 splice acceptor site
    R2020:Dock7 UTSW 4 98959101 missense possibly damaging 0.80
    R2092:Dock7 UTSW 4 99009308 missense probably benign 0.25
    R2293:Dock7 UTSW 4 98966369 missense probably damaging 1.00
    R2424:Dock7 UTSW 4 98945307 nonsense probably null
    R3767:Dock7 UTSW 4 98970829 missense probably benign 0.00
    R3768:Dock7 UTSW 4 98970829 missense probably benign 0.00
    R3769:Dock7 UTSW 4 98970829 missense probably benign 0.00
    R3770:Dock7 UTSW 4 98970829 missense probably benign 0.00
    R3917:Dock7 UTSW 4 99016685 missense possibly damaging 0.56
    R3943:Dock7 UTSW 4 98992431 missense probably benign 0.00
    R4021:Dock7 UTSW 4 99003920 splice acceptor site probably null
    R4073:Dock7 UTSW 4 99008059 missense not run
    R4170:Dock7 UTSW 4 98966401 missense not run
    R4180:Dock7 UTSW 4 99016736 missense not run
    R4261:Dock7 UTSW 4 99003886 missense not run
    R4321:Dock7 UTSW 4 99072454 missense not run
    X0025:Dock7 UTSW 4 98970781 splice donor site probably benign
    X0027:Dock7 UTSW 4 99003853 missense possibly damaging 0.73

    Phenotype:

    Homozygous phenotype: The moonlight mutation was induced by ENU mutagenesis on the C57BL/6J background, and was discovered in G3 animals. Moonlight mice have a slightly lightened coat, white-tipped paws, and hypopigmented genitalia. Moonlight animals display an occasional belly spot similar to splotch2 mutants, and sometimes a white-tipped tail. Moonlight females are poor mothers, and their pups require fostering.

    Heterozygous phenotype: No phenotype

    Mammalian Phenotype Terms:
    Allelic Composition: Dock7mnlt/Dock7mnlt (Genetic Background: C57BL/6J-Dock7mnlt )

    Strain of Origin: C57BL/6J

    Strain genetic background: C57BL/6J

    Strain Development: The moonlight mutation was induced by ENU mutagenesis on the C57BL/6J background, and was discovered in G3 animals. All subsequent crosses to maintain strain were on a C57BL/6J background.

    Suggested Control Mice:

    • Wildtype littermates

    • Cell Biology
    • Developmental Biology
    • Neurobiology
    • Sensorineural
    • Pigmentation

    Donor: Bruce Beutler, M.D., The Scripps Research Institute

    Primary Reference:

    • Blasius AL; Brandl K; Crozat K; Xia Y; Khovananth K; Krebs P; Smart NG; Zampolli A; Ruggeri ZM; Beutler BA, Mice with mutations of Dock7 have generalized hypopigmentation and white-spotting but show normal neurological function., Proc Natl Acad Sci U S A 2009 Feb 24;106(8):2706-11 (Medline PMID: 19202056)
    • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

    Colony and Husbandry Information

    Homozygous females have problems nurturing young; pups require fostering. Because of poor homozygous female breeding, it is best to breed homozygous males with heterozygous females.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    030498-UCD-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    030498-UCD-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2
    030498-UCD-EMBRYOCryo-preserved embryos
    $1,038.00
    Non-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.