Strain Detail Sheet

Strain Name    :

C57BL/6J-Dock7mnlt/Mmucd

Stock Number :

030498-UCD

Other Names   :

moonlight, C57BL/6J-Dock7mnlt/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Dock7mnlt
Name: moonlight
Alteration at locus: Chemically Induced
Gene Symbol: Dock7
Name: dedicator of cytokinesis 7
Chromosome: 4
Alteration at locus: Chemically Induced

Genetic Alterations:
The moonlight mutation was mapped to Chromosome 4. Sequencing of the critical region found a deletion spanning exons 22-28 (of 48 total exons) of the Dock7 gene (positions 118824 to137848 occurring in the middle of introns 22 and 29 in the Genbank genomic region NC_000070 for linear genomic DNA sequence of Dock7). If the donor and acceptor splice sites of introns 22 and 29 are still used, amino acids 866-1173 of the DOCK7 protein would be deleted, and a frameshift and insertion of 30 aberrant amino acids before a premature stop codon would occur.

Genotype Determination:

    ES Cell Line: Not Applicable

    Strain Description [Including phenotype, strain background, strain development and suggested control mice]

    Phenotype

    Homozygous phenotype: The moonlight mutation was induced by ENU mutagenesis on the C57BL/6J background, and was discovered in G3 animals. Moonlight mice have a slightly lightened coat, white-tipped paws, and hypopigmented genitalia. Moonlight animals display an occasional belly spot similar to splotch2 mutants, and sometimes a white-tipped tail. Moonlight females are poor mothers, and their pups require fostering.

    Heterozygous phenotype: No phenotype


    Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
          assigned by genotype
    Dock7mnlt/Dock7mnlt
            C57BL/6J-Dock7<mnlt>
    • behavior/neurological phenotype
      • behavior/neurological phenotype (MGI Ref ID J:146458)
        • homozygous mutant and wild type C57BL/6J mice exhibit similar locomotor activity; response in the tail-suspension test for depressive behavior; learning as measured by habituation of object exploration; working memory as measured using the Y maze; anxiety-like behavior in a light-dark transfer test; and preference for social familiarity/novelty
        • abnormal maternal behavior (MGI Ref ID J:146458)
          • homozygous mutant females are poor mothers, and their pups must be fostered to survive to weaning; this is not true of misty (Dock7m) mothers
        • abnormal social investigation (MGI Ref ID J:146458)
          • female moonlight mice show a tendency, though not statistically significant (P=0.0619), toward decreased social interaction
    • limbs/digits/tail phenotype
      • abnormal digit pigmentation (MGI Ref ID J:146458)
        • the distal extremities of the paws of homozygous mutant mice invariably lack pigment
      • non-pigmented tail tip (MGI Ref ID J:146458)
        • variable; incompletely penetrant
    • pigmentation phenotype
      • abnormal digit pigmentation (MGI Ref ID J:146458)
        • the distal extremities of the paws of homozygous mutant mice invariably lack pigment
      • abnormal skin pigmentation (MGI Ref ID J:146458)
        • hypopigmentation of external genitalia
        • non-pigmented tail tip (MGI Ref ID J:146458)
          • variable; incompletely penetrant
      • belly spot (MGI Ref ID J:146458)
        • white spotting, variable in extent, may be present on the belly; penetrance of this phenotype is incomplete
      • diluted coat color (MGI Ref ID J:146458)
    • homeostasis/metabolism phenotype
      • homeostasis/metabolism phenotype (MGI Ref ID J:146458)
        • the bleeding time of homozygous moonlight mice is similar to that of normal controls, indicating normal function of platelet dense granules; this is in contrast to the prolonged bleeding time reported for misty (Dock7m) homozygotes
    • immune system phenotype
      • immune system phenotype (MGI Ref ID J:146458)
        • in vivo cytotoxic efficiency of natural killer (NK) cells vs. target cells is normal
        • resistance of homozygous mutant mice to infection by mouse cytomegalovirus (MCMV), a measure of NK cell function, is similar to that of resistant C57BL/6J control mice as determined by survival and by viral clearance from the spleen at 5 days post infection
        • in vivo cytotoxic efficiency of T lymphocytes vs. target cells is normal
    • hearing/vestibular/ear phenotype
      • hearing/vestibular/ear phenotype (MGI Ref ID J:146458)
        • homozygous moonlight mice are able to hear
    • vision/eye phenotype
      • vision/eye phenotype (MGI Ref ID J:146458)
        • homozygous moonlight mice can see
    • integument phenotype
      • abnormal skin pigmentation (MGI Ref ID J:146458)
        • hypopigmentation of external genitalia
        • non-pigmented tail tip (MGI Ref ID J:146458)
          • variable; incompletely penetrant
      • belly spot (MGI Ref ID J:146458)
        • white spotting, variable in extent, may be present on the belly; penetrance of this phenotype is incomplete
      • diluted coat color (MGI Ref ID J:146458)

    Strain of Origin: C57BL/6J

    Strain genetic background: C57BL/6J

    Strain Development: The moonlight mutation was induced by ENU mutagenesis on the C57BL/6J background, and was discovered in G3 animals. All subsequent crosses to maintain strain were on a C57BL/6J background.

    Suggested Control Mice:

    • Wildtype littermates

    Research Applications

    • Cell Biology
    • Developmental Biology
    • Neurobiology
    • Sensorineural
    • Pigmentation

    Strain Origin

    Donor: Bruce Beutler, M.D., The Scripps Research Institute

    Primary Reference:

    • Blasius AL; Brandl K; Crozat K; Xia Y; Khovananth K; Krebs P; Smart NG; Zampolli A; Ruggeri ZM; Beutler BA, Mice with mutations of Dock7 have generalized hypopigmentation and white-spotting but show normal neurological function., Proc Natl Acad Sci U S A 2009 Feb 24;106(8):2706-11 (Medline PMID: 19202056)
    • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

    Colony and Husbandry Information

    Special Considerations

    Homozygous females have problems nurturing young; pups require fostering. Because of poor homozygous female breeding, it is best to breed homozygous males with heterozygous females.

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    030498-UCD-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    030498-UCD-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2
    030498-UCD-EMBRYOCryo-preserved embryos
    $1,038.00
    Non-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

    This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

    Generated: 04/23/2014