Strain Detail Sheet

Strain Name    :

STOCK Sox4tm1Cle/Mmmh

Stock Number :

030510-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Sox4tm1Cle
Name: targeted mutation 1, Hans Clevers
Alteration at locus: Knockout
Gene Symbol: Sox4
Name: SRY (sex determining region Y)-box 4
Chromosome: 13
Alteration at locus: Knockout

Genetic Alterations:
5' end of the coding sequence of Sox4 replaced by neo

Genotype Determination:

ES Cell Line: E14

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Embryonic lethality at ED 14-15 from cardiac outflow tract defects (persistant truncus arteriosus, semilunar valve abnormalities)

Heterozygous phenotype: None


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Sox4tm1Cle/Sox4tm1Cle
        involves: 129P2/OlaHsd * C57BL/6
  • mortality/aging
    • complete lethality throughout fetal growth and development (MGI Ref ID J:32741)
      • homozygous embryos died at E14
  • cardiovascular system phenotype
    • abnormal aorticopulmonary septum morphology (MGI Ref ID J:32741)
      • impaired development of the aortopulmonary septum
    • abnormal blood circulation (MGI Ref ID J:32741)
      • oscillating blood flow
    • abnormal conotruncal ridge morphology (MGI Ref ID J:51464)
      • at E13 and E14, the two endocardial ridges were always present, but the number and position of intercalated ridges between them differed from controls
      • failure of conotruncal ridge closure (MGI Ref ID J:32741)
        • abnormal endocardial ridges; never fused and failed to develop into semiluminar valves
    • abnormal coronary artery morphology (MGI Ref ID J:51464)
      • at E13, only 25% of mutant ED13 hearts develop coronary arteries
    • abnormal semilunar valve morphology (MGI Ref ID J:32741)
      • dysplasia of the valves
    • increased heart rate (MGI Ref ID J:32741)
    • perimembraneous ventricular septal defect (MGI Ref ID J:32741)
      • septation defe ct affected the outlet portion of the ventricles and the great arteries
    • persistent right dorsal aorta (MGI Ref ID J:51464)
      • both dorsal aortae were still present in 7 of 8 E13 mutant embryos, whereas the right dorsal aorta had disappeared in all control littermates
      • by E14 the right dorsal aorta, if present, had diminished greatly in diameter in mutant embryos
  • hematopoietic system phenotype
    • abnormal B cell differentiation (MGI Ref ID J:32741)
      • very few mature B cells are produced in irradiated mice reconstituted with mutant fetal liver cells
      • absent pre-B cells (MGI Ref ID J:32741)
        • Cd43-B220+ pre-B cells were nearly absent
      • decreased pro-B cell number (MGI Ref ID J:32741)
        • CD43+B220+ pro-B cell numbers were reduced
  • homeostasis/metabolism phenotype
    • hydrops fetalis (MGI Ref ID J:32741)
      • generalized edema apparent at E14
  • immune system phenotype
    • abnormal B cell differentiation (MGI Ref ID J:32741)
      • very few mature B cells are produced in irradiated mice reconstituted with mutant fetal liver cells
      • absent pre-B cells (MGI Ref ID J:32741)
        • Cd43-B220+ pre-B cells were nearly absent
      • decreased pro-B cell number (MGI Ref ID J:32741)
        • CD43+B220+ pro-B cell numbers were reduced
  • digesti ve/alimentary phenotype
    • tracheoesophageal fistula (MGI Ref ID J:51464)
      • observed in 4 mutant embryos
  • respiratory system phenotype
    • tracheoesophageal fistula (MGI Ref ID J:51464)
      • observed in 4 mutant embryos

Strain of Origin: C57BL/6

Strain genetic background: outbred onto CD1

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Developmental Biology
  • Neurobiology
  • Research Tools

Strain Origin

Donor: Kathryn Maschhoff, M.D., Ph.D., Children's Hospital of Philadelphia

Primary Reference:

  • Schilham MW, Oosterwegel MA, Moerer P, Ya J, de Boer PA, van de Wetering M, Verbeek S, Lamers WH, Kruisbeek AM, Cumano A, Clevers H. Defects in cardiac outflow tract formation and pro-B-lymphocyte expansion in mice lacking Sox-4. Nature. 1996 Apr 25;380(6576):711-4. (Medline PMID: 8614465 )
  • Ya J, Schilham MW, de Boer PA, Moorman AF, Clevers H, Lamers WH. Sox4-deficiency syndrome in mice is an animal model for common trunk. Circ Res. 1998 Nov 16;83(10):986-94. (Medline PMID: 9815146)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030510-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030510-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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