Strain Name:
B6.129-Il15ratm1Wjl/Mmnc
Stock Number:
030514-UNC
Citation ID:
RRID:MMRRC_030514-UNC

Strain Information

Il15ratm1Wjl
Name: interleukin 15 receptor, alpha chain; targeted mutation 1, Warren J Leonard
Synonyms: IL-15Ralphaext/IL-2Ralphaint
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knock-In
Il15ra
Name: interleukin 15 receptor, alpha chain
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Knock-In
NCBI: 16169
HGNC: HGNC:5978
Homologene: 1650
Genetic Alterations
The targeting vector was linearized with Not I, electroporated into strain 129 embryonic stem (ES) cells, and selected with G418.36 Generation of chimeric IL-15R alpha/IL-2R alpha knock-in mice: The targeting vector was linearized with Not I, electroporated into strain 129 embryonic stem (ES) cells, and selected with G418.36 Genomic DNA from 192 ES clones was digested with Hpa I and Southern blotted to yield a 7.5 kb band for the WT allele vs. a 9.3 kb band when the PGK-neo cassette was inserted by homologous recombination.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not specified
    Phenotype
    Homozygous: No phenotype

    Heterozygous: No phenotype
    Mammalian Phenotype Terms
    Allelic Composition: Il15ratm1Wjl/Il15ratm1Wjl (Genetic Background: involves: 129 * C57BL/6 )

    MeSH Terms
    • Adoptive Transfer/veterinary
    • Animals
    • Antigen Presentation/physiology
    • CD8-Positive T-Lymphocytes/cytology
    • CD8-Positive T-Lymphocytes/metabolism
    • CD8-Positive T-Lymphocytes/physiology
    • Cell Proliferation
    • Cells, Cultured
    • Cytoplasm/metabolism
    • Female
    • Interferon-gamma/metabolism
    • Interleukin-15/metabolism
    • Interleukin-15 Receptor alpha Subunit/chemistry
    • Interleukin-15 Receptor alpha Subunit/genetics
    • Interleukin-15 Receptor alpha Subunit/metabolism
    • Interleukin-15 Receptor alpha Subunit/physiology
    • Male
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Natural Killer T-Cells/cytology
    • Natural Killer T-Cells/metabolism
    • Natural Killer T-Cells/physiology
    • Protein Structure, Tertiary/physiology
    • Transfection
    Strain Development
    Generation of Il15ratm1Wjl Knock-In Mice: The targeting vector was linearized with Not I, electroporated into strain 129 embryonic stem (ES) cells, and selected with G418.36 Generation of chimeric IL-15R alpha/IL-2R alpha knock-in mice: The targeting vector was linearized with Not I, electroporated into strain 129 embryonic stem (ES) cells, and selected with G418.36 Genomic DNA from 192 ES clones was digested with Hpa I and Southern blotted to yield a 7.5 kb band for the WT allele vs. a 9.3 kb band when the PGK-neo cassette was inserted by homologous recombination. Homologous recombination was confirmed by digesting the ES cell DNA with Xho I and Southern blotting with probe B. ES cell clones with a chimeric Il15ratm1Wjl knock-in allele were injected into C57BL/6 blastocysts, resulting male knock-in mice were bred to C57BL/6 females, and progeny were analyzed for germ line transmission of the knock-in allele by PCR and Southern blotting. Southern blotting after Xho I digestion yielded a band of 14.6 kb for the WT allele and 4.4 kb for the knock-in allele after homologous recombination. To avoid effects that might result from the neomycin cassette, mice carrying the knock-in allele were mated to EIIa-Cre transgenic mice, allowing excision of this LoxP-flanked cassette, yielding a 2.5 kb band, as confirmed by Southern blotting. Mice heterozygous for the knock-in allele but lacking the neomycin cassette were inter-crossed to generate Il15ratm1Wjl knock-in mice.
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Immunology and Inflammation
    Donor
    Warren Leonard, M.D., National Heart, Lung, and Blood Institute (NHLBI)
    Primary Reference
    Wu Z, Xue HH, Bernard J, Zeng R, Issakov D, Bollenbacher-Reilley J, Belyakov IM, Oh S, Berzofsky JA, Leonard WJ. The IL-15 receptor {alpha} chain cytoplasmic domain is critical for normal IL-15Ralpha function but is not required for trans-presentation. Blood. 2008 Dec 1;112(12):4411-9. Epub 2008 Sep 16. (Medline PMID: 18796634)

    Colony and Husbandry Information

    Pathogen Free Facility (standard)

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    030514-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.