Strain Name:
B6J.129P2-Tnfrsf10btm1Wsed/Mmmh
Stock Number:
030532-MU
Citation ID:
RRID:MMRRC_030532-MU
Other Names:
DR5-/-

Strain Information

Tnfrsf10btm1Wsed
Name: tumor necrosis factor receptor superfamily, member 10b; targeted mutation 1, Wafik S El-Diery
Synonyms: DR5-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
Tnfrsf10b
Name: tumor necrosis factor receptor superfamily, member 10b
Synonyms: Killer/Dr5, Trail Receptor, TRICK2B, TRAILR2, TRICKB, TRAIL-R2, DR5, TRICK2A, KILLER, Ly98
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Knockout
NCBI: 21933
VEGA: 14
Homologene: 129806
Genetic Alterations
Knockout of Tnfrsf10b (MGI:1341090), where exons 3 and 4 and part of 5 were replaced with a PGK-neo cassette in the reverse orientation, causing a downstream frameshift. The deleted sequence encompassed the extracellular domain of the gene and encoded the cysteine-rich repeats.
Genotype Determination
ES Cell Line
E14K derived from 129P2/OlaHsd
Phenotype
Homozygous: Same as wild-type.

Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Tnfrsf10btm1Wsed/Tnfrsf10btm1Wsed (Genetic Background: involves: 129P2/OlaHsd )

MeSH Terms
  • Animals
  • Apoptosis/physiology
  • Apoptosis Regulatory Proteins
  • Caspase 3
  • Caspases/analysis
  • Caspases/metabolism
  • Chorion/metabolism
  • DNA Damage
  • Decidua/metabolism
  • Doxorubicin/pharmacology
  • Embryo, Mammalian/cytology
  • Embryo, Mammalian/metabolism
  • Embryonic Development/genetics
  • Female
  • Fibroblasts/cytology
  • Fibroblasts/drug effects
  • Gene Expression
  • Gene Targeting
  • Male
  • Membrane Glycoproteins/pharmacology
  • Membrane Glycoproteins/physiology
  • Mice
  • Mice, Knockout
  • Radiation, Ionizing
  • Receptors, TNF-Related Apoptosis-Inducing Ligand
  • Receptors, Tumor Necrosis Factor/genetics
  • Receptors, Tumor Necrosis Factor/physiology
  • Sequence Deletion/genetics
  • Spleen/chemistry
  • Spleen/cytology
  • Spleen/radiation effects
  • TNF-Related Apoptosis-Inducing Ligand
  • Thymus Gland/chemistry
  • Thymus Gland/cytology
  • Thymus Gland/radiation effects
  • Tumor Necrosis Factor-alpha/pharmacology
  • Tumor Necrosis Factor-alpha/physiology
  • Alkylating Agents/toxicity
  • Bronchitis/chemically induced
  • Bronchitis/genetics
  • Bronchitis/metabolism
  • Bronchitis/pathology
  • Cell Line, Tumor
  • Cell Transformation, Neoplastic/drug effects
  • Cell Transformation, Neoplastic/genetics
  • Cell Transformation, Neoplastic/metabolism
  • Cell Transformation, Neoplastic/pathology
  • Cell Transformation, Neoplastic/radiation effects
  • Chronic Disease
  • Diethylnitrosamine/toxicity
  • Humans
  • Inflammation/chemically induced
  • Inflammation/genetics
  • Inflammation/metabolism
  • Inflammation/pathology
  • Liver Neoplasms/chemically induced
  • Liver Neoplasms/genetics
  • Liver Neoplasms/metabolism
  • Liver Neoplasms/pathology
  • Lymphoma/chemically induced
  • Lymphoma/genetics
  • Lymphoma/metabolism
  • Lymphoma/pathology
  • Pneumonia/chemically induced
  • Pneumonia/genetics
  • Pneumonia/metabolism
  • Pneumonia/pathology
  • Receptors, TNF-Related Apoptosis-Inducing Ligand/genetics
  • Receptors, TNF-Related Apoptosis-Inducing Ligand/metabolism
  • Transcription Factor RelA/genetics
  • Transcription Factor RelA/metabolism
  • Tumor Suppressor Proteins/genetics
  • Tumor Suppressor Proteins/metabolism
  • Whole-Body Irradiation/adverse effects
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Hematology
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
  • Virology
Donor
Wafik S. El-Deiry, M.D., Ph.D., University of Pennsylvania
Primary Reference
Finnberg N, Gruber JJ, Fei P, Rudolph D, Bric A, Kim SH, Burns TF, Ajuha H, Page R, Wu GS, Chen Y, McKenna WG, Bernhard E, Lowe S, Mak T, El-Deiry WS. DR5 knockout mice are compromised in radiation-induced apoptosis. Mol Cell Biol. 2005 Mar;25(5):2000-13. (Medline PMID: 15713653)

Finnberg N, Klein-Szanto AJ, El-Deiry WS. TRAIL-R deficiency in mice promotes susceptibility to chronic inflammation and tumorigenesis. J Clin Invest. 2008 Jan;118(1):111-23. (Medline PMID: 18079962)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Bred to Homozygosity
No

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030532-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.