Strain Detail Sheet

Strain Name    :

B6.129P2-Tnfrsf10btm1Wsed/Mmmh

Stock Number :

030532-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Tnfrsf10btm1Wsed
Name: targeted mutation 1, Wafik S El-Diery
Alteration at locus: Knockout
Gene Symbol: Tnfrsf10b
Name: tumor necrosis factor receptor superfamily, member 10b
Chromosome: 14
Alteration at locus: Knockout

Genetic Alterations:

Genotype Determination:

ES Cell Line: Not specified derived from B6;129P2

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: None

Heterozygous phenotype: None


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Tnfrsf10btm1Wsed/Tnfrsf10btm1Wsed
        involves: 129P2/OlaHsd
  • cellular phenotype
    • decreased cellular sensitivity to gamma-irradiation (MGI Ref ID J:96816)
      • after exposure to 5 Gy, decreased apoptosis is seen in the thymus, white pulp if the spleen, liver, Peyer's patches of the ileum, and white matter of the brain; however apoptosis was similar to wild-type in the stomach, proximal colon, kidney, lung, and esophagus
      • injection of TNFSF10 in combination with radiation exposure increased apoptosis in the white pulp of the spleen in wild-type mice but not in homozygous mutants
  • homeostasis/metabolism phenotype
    • decreased physiological sensitivity to xenobiotic (MGI Ref ID J:96816)
      • dexamethasone-induced apoptosis is decreased in the thymus, but not in the spleen
  • immune system phenotype
    • enlarged thymus (MGI Ref ID J:96816)
  • hematopoietic system phenotype
    • enlarged thymus (MGI Ref ID J:96816)

Strain of Origin: mixed C57BL/6 and 129Sv

Strain genetic background: C57BL/6

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cancer
  • Hematology
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
  • Virology

Strain Origin

Donor: Wafik S. El-Deiry, M.D., Ph.D., University of Pennsylvania

Primary Reference:

  • Finnberg N, Gruber JJ, Fei P, Rudolph D, Bric A, Kim SH, Burns TF, Ajuha H, Page R, Wu GS, Chen Y, McKenna WG, Bernhard E, Lowe S, Mak T, El-Deiry WS. DR5 knockout mice are compromised in radiation-induced apoptosis. Mol Cell Biol. 2005 Mar;25(5):2000-13. (Medline PMID: 15713653 )
  • Finnberg N, Klein-Szanto AJ, El-Deiry WS. TRAIL-R deficiency in mice promotes susceptibility to chronic inflammation and tumorigenesis. J Clin Invest. 2008 Jan;118(1):111-23. (Medline PMID: 18079962)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030532-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
030532-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/20/2014