Strain Name:
129S-Gt(ROSA)26Sortm1(Tfrc*)Nca/Mmnc
Stock Number:
030618-UNC
Citation ID:
RRID:MMRRC_030618-UNC

Strain Information

Gt(ROSA)26Sortm1(Tfrc*)Nca
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Nancy C Andrews
Synonyms: ROSA26Tfr1R654A
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
NCBI: 14910
Genetic Alterations
The entire coding sequence of mouse Tfr1 with a R654A mutation was placed under the control of the ROSA26 locus.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    J1 derived from 129S4/SvJae
    Phenotype
    Homozygous: The mice overload iron in tissue and have increased blood iron parameters. The phenotype is not as severe as Hfe-/- or Hjv-/- animals.

    Heterozygous: A milder form of the homozygous phenotype.
    Mammalian Phenotype Terms
    Allelic Composition: Gt(ROSA)26Sortm1(Tfrc*)Nca/Gt(ROSA)26Sortm1(Tfrc*)Nca (Genetic Background: involves: 129S4/SvJae * FVB/N )

    MeSH Terms
    • Animals
    • Antimicrobial Cationic Peptides/genetics
    • Antimicrobial Cationic Peptides/metabolism
    • Binding Sites
    • Erythropoiesis
    • Gene Expression Regulation
    • Genotype
    • Hemochromatosis Protein
    • Hepcidins
    • Histocompatibility Antigens Class I/genetics
    • Histocompatibility Antigens Class I/metabolism
    • Homeostasis
    • Iron/deficiency
    • Iron/metabolism
    • Iron Overload/genetics
    • Iron Overload/metabolism
    • Iron Overload/physiopathology
    • Liver/metabolism
    • Membrane Proteins/genetics
    • Membrane Proteins/metabolism
    • Mice
    • Mice, Knockout
    • Mice, Transgenic
    • Mutation, Missense
    • Phenotype
    • Protein Binding
    • RNA, Messenger/metabolism
    • Receptors, Transferrin/genetics
    • Receptors, Transferrin/metabolism
    • Signal Transduction/genetics
    • Transferrin/metabolism
    Strain Development
    Mutated Tfr1 cDNA targeted to the ROSA26 locus
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Hematology
    • Internal/Organ
    • Metabolism
    • Models for Human Disease
    Donor
    Paul J. Schmidt, Ph.D., Boston Children's Hospital
    Primary Reference
    Schmidt PJ, Toran PT, Giannetti AM, Bjorkman PJ, Andrews NC. The transferrin receptor modulates Hfe-dependent regulation of hepcidin expression. Cell Metab. 2008 Mar;7(3):205-14. (Medline PMID: 18316026)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    030618-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    030618-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.