Strain Name:
C57BL/6J-Tg(Ttr-Hfe)1Nca/Mmnc
Stock Number:
030621-UNC
Citation ID:
RRID:MMRRC_030621-UNC

Strain Information

Hfe
Name: homeostatic iron regulator
Synonyms: MR2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15216
HGNC: HGNC:4886
Homologene: 88330
Ttr
Name: transthyretin
Synonyms: prealbumin
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 22139
VEGA: 18
Homologene: 317
Tg(Ttr-Hfe)1Nca
Name: transgene insertion 1, Nancy C Andrews
Synonyms: Tfr-Hfe
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The Ttr promoter was used to drive Hfe expression in a hepatocyte-specific manner. The Hfe cDNA was inserted into the second intron of Ttr.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Homozygous: Unknown - transgene is carried as a single copy.
    Hemizygous: Mice are highly anemic.
    MeSH Terms
    • Animals
    • Antimicrobial Cationic Peptides/genetics
    • Antimicrobial Cationic Peptides/metabolism
    • Binding Sites
    • Erythropoiesis
    • Gene Expression Regulation
    • Genotype
    • Hemochromatosis Protein
    • Hepcidins
    • Histocompatibility Antigens Class I/genetics
    • Histocompatibility Antigens Class I/metabolism
    • Homeostasis
    • Iron/deficiency
    • Iron/metabolism
    • Iron Overload/genetics
    • Iron Overload/metabolism
    • Iron Overload/physiopathology
    • Liver/metabolism
    • Membrane Proteins/genetics
    • Membrane Proteins/metabolism
    • Mice
    • Mice, Knockout
    • Mice, Transgenic
    • Mutation, Missense
    • Phenotype
    • Protein Binding
    • RNA, Messenger/metabolism
    • Receptors, Transferrin/genetics
    • Receptors, Transferrin/metabolism
    • Signal Transduction/genetics
    • Transferrin/metabolism
    Strain Development
    Founder mice and subsequent generations were bred to C57BL/6J.
    Suggested Control Mice

    Wildtype littermates or C57BL/6J mice

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cell Biology
    • Hematology
    • Internal/Organ
    • Metabolism
    • Models for Human Disease
    Donor
    Paul J Schmidt, Ph.D., Boston Children's Hospital .
    Primary Reference
    Schmidt PJ, Toran PT, Giannetti AM, Bjorkman PJ, Andrews NC. The transferrin receptor modulates Hfe-dependent regulation of hepcidin expression. Cell Metab. 2008 Mar;7(3):205-14. (Medline PMID: 18316026)

    Colony and Husbandry Information

    The transgene is generally carried by the male due to the resultant anemia of animals expressing the transgene. However, females carrying the transgenic integration are viable and fertile. As a result of being anemic, females carrying the transgene are less able to support large numbers of pups. As a result of being anemic, the transgene is carried by the male and bred to a wildtype female.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    030621-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.