Strain Name    : B6.Cg-Tg(RP23-326M22)760She/Mmmh

Stock Number : 030622-MU

Gene Information

(provided by MGI)
Transgene: Tg(RP23-326M22)760She
Name: transgene insertion 760, Sarah Elsea
Alteration at locus: Transgenic (BAC)
Transgene: Rai1
Name: retinoic acid induced 1
Alteration at locus: Transgenic (BAC)
Genetic Alterations:
randomly inserted BAC transgenic, 2 copies inserted

Genotype Determination:

ES Cell Line:

Phenotype

Homozygous phenotype: Highly anxious/hyperkinetic to the extent they are difficult to evaluate; delayed growth >females than males; neurological deficits. Further, analyses of homozygous transgenic mice revealed a dosage-dependent exacerbation of the phenotype, including extreme growth retardation, severe neurological deficits, and increased hyperactivity. Rai1 dosage has major consequences on molecular processes involved in growth, development, and neurological and behavioral functions

Hemizygous phenotype: Growth retardation, increased locomotor activity, and abnormal anxiety- related behavior compared to wild type littermates; altered gait with short strides and long sways, impaired ability on a cage-top hang test, decreased forelimb grip strength, and a dominant social behavior.

Mammalian Phenotype Terms:
Allelic Composition: Tg(RP23-326M22)760She/Tg(RP23-326M22)760She (Genetic Background: B6.Cg-Tg(RP23-326M22)760She )

Allelic Composition: Tg(RP23-326M22)760She/0 (Genetic Background: B6.Cg-Tg(RP23-326M22)760She )

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: BAC (RP23-326M22) transgenic mouse created at U of Michigan transgenic core. After using a modified Qiagen DNA isolation protocol for low-copy plasmids (Qiagen Inc, Valencia, CA), 0.5-1 ng/μl of purified, Not1-linearized BAC DNA was microinjected into the fertilized mouse eggs from two separate crosses: (C57BL/6J x SJL) F1 and (C57BL/6J x SJL) F1 or C57BL/6J x (C57BL/6J x SJL) F1, and eggs were transferred to recipient mothers. Three weeks following the microinjection, founder pups were born, genotyped, and later bred to C57BL/6J females. Two male founders, mice 760 and 775, showed single, intact BAC site insertion into the genome and germ line transmission of the BAC transgene. Transgenic lines were established and maintained by backcrossing to C57BL/6J mice for more than 8 generations (>99% C57BL/6J background). The BAC-transgenic mice are named according to the International Committee on Standardized Genetic Nomenclature for Mice.

Rai1 transgenic male founders were bred with C57BL/6J female mice obtained from the JAX

Suggested Control Mice:

  • Wildtype littermates

  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Sensorineural

Donor: Sarah Elsea, Ph.D., Virginia Commonwealth University

Primary Reference:

Girirajan S, Patel N, Slager RE, Tokarz ME, Bucan M, Wiley JL, Elsea SH. How much is too much? Phenotypic consequences of Rai1 overexpression in mice. Eur J Hum Genet. 2008 Aug;16(8):941-54. Epub 2008 Feb 20. (Medline PMID: 18285828)

Colony and Husbandry Information

Donor always used a high fat breeder's diet, no special housing utilized.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
030622-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
030622-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.