Name: transgene insertion 760, Sarah Elsea
Alteration at locus: Transgenic (BAC)
Name: retinoic acid induced 1
Alteration at locus: Transgenic (BAC)
ES Cell Line:
Homozygous phenotype: Highly
anxious/hyperkinetic to the extent they are difficult to evaluate;
delayed growth >females than males; neurological deficits. Further,
analyses of homozygous transgenic mice revealed a dosage-dependent
exacerbation of the phenotype, including extreme growth retardation,
severe neurological deficits, and increased hyperactivity. Rai1 dosage
has major consequences on molecular processes involved in growth,
development, and neurological and behavioral functions
Hemizygous phenotype: Growth retardation, increased locomotor activity, and abnormal anxiety- related behavior compared to wild type littermates; altered gait with short strides and long sways, impaired ability on a cage-top hang test, decreased forelimb grip strength, and a dominant social behavior.
Strain of Origin: C57BL/6J
Strain genetic background: C57BL/6J
Strain Development: BAC (RP23-326M22) transgenic mouse
created at U of Michigan transgenic core. After using a modified Qiagen
DNA isolation protocol for low-copy plasmids (Qiagen Inc, Valencia, CA),
0.5-1 ng/μl of purified, Not1-linearized BAC DNA was microinjected
into the fertilized mouse eggs from two separate crosses: (C57BL/6J x
SJL) F1 and (C57BL/6J x SJL) F1 or C57BL/6J x (C57BL/6J x SJL) F1, and
eggs were transferred to recipient mothers. Three weeks following the
microinjection, founder pups were born, genotyped, and later bred to
C57BL/6J females. Two male founders, mice 760 and 775, showed single,
intact BAC site insertion into the genome and germ line transmission of
the BAC transgene. Transgenic lines were established and maintained by
backcrossing to C57BL/6J mice for more than 8 generations (>99% C57BL/6J
background). The BAC-transgenic mice are named according to the
International Committee on Standardized Genetic Nomenclature for Mice.
Rai1 transgenic male founders were bred with C57BL/6J female mice obtained from the JAX
Suggested Control Mice:
Donor: Sarah Elsea, Ph.D., Virginia Commonwealth University
Girirajan S, Patel N, Slager RE, Tokarz ME, Bucan M, Wiley JL, Elsea SH. How much is too much? Phenotypic consequences of Rai1 overexpression in mice. Eur J Hum Genet. 2008 Aug;16(8):941-54. Epub 2008 Feb 20. (Medline PMID: 18285828)
Donor always used a high fat breeder's diet, no special housing utilized.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.