Strain Detail Sheet

Strain Name    :

B6.Cg-Tg(RP23-326M22)760She/Mmmh

Stock Number :

030622-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(RP23-326M22)760She
Name: transgene insertion 760, Sarah Elsea
Alteration at locus: Transgenic (BAC)
Transgene: Rai1
Name: retinoic acid induced 1
Alteration at locus: Transgenic (BAC)

Genetic Alterations:
randomly inserted BAC transgenic, 2 copies inserted

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Highly anxious/hyperkinetic to the extent they are difficult to evaluate; delayed growth >females than males; neurological deficits. Further, analyses of homozygous transgenic mice revealed a dosage-dependent exacerbation of the phenotype, including extreme growth retardation, severe neurological deficits, and increased hyperactivity. Rai1 dosage has major consequences on molecular processes involved in growth, development, and neurological and behavioral functions

Hemizygous phenotype: Growth retardation, increased locomotor activity, and abnormal anxiety- related behavior compared to wild type littermates; altered gait with short strides and long sways, impaired ability on a cage-top hang test, decreased forelimb grip strength, and a dominant social behavior.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Tg(RP23-326M22)760She/-
        B6.Cg-Tg(RP23-326M22)760She
  • behavior/neurological phenotype
    • abnormal locomotor activity (MGI Ref ID J:161765)
      • increased motor activity as determined by number of interruptions to photo cell beams
      • abnormal gait (MGI Ref ID J:161765)
        • mice exhibit a shorter, wider gait and an increased sway distance
        • short stride length (MGI Ref ID J:161765)
      • decreased vertical activity (MGI Ref ID J:161765)
        • decreased rearing behavior at 10 and 20 weeks, although behavior is similar to controls at 5 weeks
      • hyperactivity (MGI Ref ID J:161765)
        • mice exhibit overtly active, wild running behavior
        • mice exhibit increased motor activity at 5 and 10 weeks, but behave similarly to controls by 20 weeks
    • abnormal posture (MGI Ref ID J:161765)
      • shortened stance
    • abnormal social/conspecific interaction (MGI Ref ID J:161765)
      • transgenics are socially dominant compared to nontransgenics as determined by the tube test
    • decreased grip strength (MGI Ref ID J:161765)
      • decreased forelimb grip strength
    • hyperresponsive to tactile stimuli (MGI Ref ID J:161765)
      • although not statistically significant, 20 week old mice exhibited hyper-responsiveness to both the hotplate and tail flick tests
    • impaired coordination (MGI Ref ID J:161765)
      • in response to the cage-top hang test, 20 week old mice easily fall off the cage-top
    • impaired righting response (MGI Ref ID J:161765)
    • increased anxiety-related response (MGI Ref ID J:161765)
      • mice spend a decreased proportion of time in the center of the open field test
  • liver/biliary system phenotype
    • abnormal hepatocyte morphology (MGI Ref ID J:161765)
      • hepatocytes contain a large nucleus altering the nucleus:cytoplasm ratio, however, levels of liver enzymes are similar to controls
  • growth/size phenotype
    • decreased body size (MGI Ref ID J:161765)
      • proportionally decreased head and body size as compared to controls
      • decreased body length (MGI Ref ID J:161765)
        • mice exhibit decreased body length up to 20 weeks of age, but are similar to controls at 30 weeks
      • decreased body weight (MGI Ref ID J:161765)
        • mice exhibit decreased body weight up to 20 weeks of age, but weight is similar to controls at 30 weeks
    • postnatal growth retardation (MGI Ref ID J:161765)
      • males exhibit greater growth retardation than females
  • reproductive system phenotype
    • reduced female fertility (MGI Ref ID J:161765)
  • integument phenotype
    • hyperresponsive to tactile stimuli (MGI Ref ID J: 161765)
      • although not statistically significant, 20 week old mice exhibited hyper-responsiveness to both the hotplate and tail flick tests
Tg(RP23-326M22)760She/Tg(RP23-326M22)760She
        B6.Cg-Tg(RP23-326M22)760She
  • behavior/neurological phenotype
    • abnormal locomotor activity (MGI Ref ID J:161765)
      • increased motor activity as determined by number of interruptions to photo cell beams
      • decreased vertical activity (MGI Ref ID J:161765)
        • reduced rearing activity
      • hyperactivity (MGI Ref ID J:161765)
        • mice are very active and difficult to handle
    • abnormal sensory capabilities/reflexes/nociception (MGI Ref ID J:161765)
      • heightened approach response (approach with a blunt object)
      • impaired righting response (MGI Ref ID J:161765)
    • decreased grip strength (MGI Ref ID J:161765)
      • decreased forelimb grip strength in comparison to hemizygotes and controls
    • impaired coordination (MGI Ref ID J:161765)
    • increased anxiety-related response (MGI Ref ID J:161765)
  • growth/size phenotype
    • decreased body weight (MGI Ref ID J:161765)
    • postnatal growth retardation (MGI Ref ID J:161765)
      • mice are slender and small
      • growth retardation in homozygotes is significantly reduced compared to hemizygotes and controls
  • reproductive system phenotype
    • reduced female fertility (MGI Ref ID J:161765)
    • reduced male fertility (MGI Ref ID J:161765)

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: BAC (RP23-326M22) transgenic mouse created at U of Michigan transgenic core. After using a modified Qiagen DNA isolation protocol for low-copy plasmids (Qiagen Inc, Valencia, CA), 0.5-1 ng/μl of purified, Not1-linearized BAC DNA was microinjected into the fertilized mouse eggs from two separate crosses: (C57BL/6J x SJL) F1 and (C57BL/6J x SJL) F1 or C57BL/6J x (C57BL/6J x SJL) F1, and eggs were transferred to recipient mothers. Three weeks following the microinjection, founder pups were born, genotyped, and later bred to C57BL/6J females. Two male founders, mice 760 and 775, showed single, intact BAC site insertion into the genome and germ line transmission of the BAC transgene. Transgenic lines were established and maintained by backcrossing to C57BL/6J mice for more than 8 generations (>99% C57BL/6J background). The BAC-transgenic mice are named according to the International Committee on Standardized Genetic Nomenclature for Mice.

Rai1 transgenic male founders were bred with C57BL/6J female mice obtained from the JAX

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Sensorineural

Strain Origin

Donor: Sarah Elsea, Ph.D., Virginia Commonwealth University

Primary Reference:

Girirajan S, Patel N, Slager RE, Tokarz ME, Bucan M, Wiley JL, Elsea SH. How much is too much? Phenotypic consequences of Rai1 overexpression in mice. Eur J Hum Genet. 2008 Aug;16(8):941-54. Epub 2008 Feb 20. (Medline PMID: 18285828)

Colony and Husbandry Information

Special Considerations

Donor always used a high fat breeder's diet, no special housing utilized.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030622-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
030622-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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