Allele Symbol: Ikbkgm1Btlr
Name: mutation 1, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Ikbkg
Name: inhibitor of kappaB kinase gamma
Alteration at locus: Chemically Induced
ES Cell Line: Not Applicable
Homozygous and Hemizygous phenotype: The panr2 ("pan- resistance") phenotype was detected in a screen of ENU-induced mutant mice for altered responses to Toll-like receptor (TLR) ligands (TLR Signaling Screen). Peritoneal macrophages from panr2 mice exhibit low tumor necrosis factor (TNF)-α production to all TLR ligands except the TLR4 ligand, lipopolysaccharide (LPS). Only a subtle reduction in TNF-α production is seen in these macrophages when stimulated with LPS. In response to the TLR3 ligand poly I:C (a dsRNA mimetic) and dsDNA, panr2 macrophages display normal type 1 interferon (IFN) production. Panr2 mice display normal natural killer (NK) cell and cytotoxic T cell (CTL) function (In vivo NK cell and CD8+ T cell cytotoxicity screen).
Although the TLR sensing defect remains as reported and hemizygous males remain azoospermatic, this mutation is no longer associated with male lethality.
Strain of Origin: C57BL/6J
Strain genetic background: C57BL/6J
Strain Development: Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice: Wild-type littermates or C57BL/6J
Donor: Bruce Beutler, M.D., The Scripps Research Institute
Donor recommends TMS antibiotic water. Hemizygous panr2 males are sterile and homozygous females do not breed. Therefore heterozygous females must be bred to wild-type males to maintain the stock. Heterozygous females are also poor breeders.
Coat color: Black
MMRRC Breeding System: Random intra-strain mating
Overall Breeding Performance: Fair
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||No||No|
|Hetero/Hemizygotes are fertile:||Yes||No||Heterozygous females are poor breeders, males are sterile|
|Age Reproductive Decline:||Unknown||Unknown|
Average litter size:
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.