Strain Name    : C57BL/6J-Ikbkgm1Btlr/Mmucd

Stock Number : 030659-UCD

Other Names   : panr2, C57BL/6J-Ikbkgm1Btlr/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Ikbkgm1Btlr
Name: mutation 1, Bruce Beutler
Chromosome: X
Alteration at locus: Chemically Induced
Gene Symbol: Ikbkg
Name: inhibitor of kappaB kinase gamma
Chromosome: X
Alteration at locus: Chemically Induced
Genetic Alterations:
The Ikbkg gene on the X chromosome of panr2 mice was sequenced, and a T to C transition was identified in exon 4 (of 10 total exons) at position 473 (using variant 1, Genbank: NM_178590). The mutation results in a leucine to proline change at amino acid 153 of the IKK-Υ or NEMO protein.

Genotype Determination:

ES Cell Line:

Additional mutations found on this strain:

AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02502:Ikbkg APN X 74443827 missense probably benign 0.00

Phenotype

Homozygous and Hemizygous phenotype: The panr2 ("pan- resistance") phenotype was detected in a screen of ENU-induced mutant mice for altered responses to Toll-like receptor (TLR) ligands (TLR Signaling Screen). Peritoneal macrophages from panr2 mice exhibit low tumor necrosis factor (TNF)-α production to all TLR ligands except the TLR4 ligand, lipopolysaccharide (LPS). Only a subtle reduction in TNF-α production is seen in these macrophages when stimulated with LPS. In response to the TLR3 ligand poly I:C (a dsRNA mimetic) and dsDNA, panr2 macrophages display normal type 1 interferon (IFN) production. Panr2 mice display normal natural killer (NK) cell and cytotoxic T cell (CTL) function (In vivo NK cell and CD8+ T cell cytotoxicity screen).

Although the TLR sensing defect remains as reported and hemizygous males remain azoospermatic, this mutation is no longer associated with male lethality.

Mammalian Phenotype Terms:
Allelic Composition: Ikbkgm1Btlr/Ikbkg+ (Genetic Background: C57BL/6J-Ikbkgm1Btlr )

  • reproductive system

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.

Suggested Control Mice: Wild-type littermates or C57BL/6J

  • Immunology and Inflammation
  • Models for Human Disease

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

  • Siggs OM, Berger M, Krebs P, Arnold CN, Eidenschenk C, Huber C, Pirie E, Smart NG, Khovananth K, Xia Y, McInerney G, Karlsson Hedestam GB, Nemazee D, Beutler B. A mutation of Ikbkg causes immune deficiency without impairing degradation of IkappaB alpha. Proc Natl Acad Sci U S A 2010 Feb 16;107(7):3046-51 (Medline PMID: 20133626)
  • Siggs OM, Smart NG, Beutler B. Record for panr2MUTAGENETIX™, B. Beutler and colleagues, The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Donor recommends TMS antibiotic water. Hemizygous panr2 males are sterile and homozygous females do not breed. Therefore heterozygous females must be bred to wild-type males to maintain the stock. Heterozygous females are also poor breeders.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: Black

Other:

MMRRC Breeding System: Random intra-strain mating

Breeding Scheme(s):

  • Heterozygous female x C57BL/6J male.

Generation:

Overall Breeding Performance: Fair

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No No
Hetero/Hemizygotes are fertile: Yes No Heterozygous females are poor breeders, males are sterile
Age Reproductive Decline: Unknown Unknown

Average litter size:

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
030659-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030659-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.