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Strain Name:
B6;129S1-Tal1tm2Wehi/Mmmh
Stock Number:
030665-MU
Citation ID:
RRID:MMRRC_030665-MU

Strain Information

Tal1tm2Wehi
Name: T cell acute lymphocytic leukemia 1; targeted mutation 2, Walter and Eliza Hall, Institute of Medical Research
Synonyms: SCL-lacZ, SCLlacZ, Scl-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knock-In
Tal1
Name: T cell acute lymphocytic leukemia 1
Synonyms: SCL/tal-1, Scl, bHLHa17, Hpt
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knock-In
NCBI: 21349
Homologene: 2400
Genetic Alterations
LacZ gene knocked into the Tal1 gene locus with deletion of exons 4 and 5.
Genotype Determination
ES Cell Line
W9.5/W95 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Phenotype
Homozygous: Homozygous lethal ~day 8.5 due to failure of vascular system development in embryo.

Heterozygous: normal
Mammalian Phenotype Terms
Allelic Composition: Tal1tm2Wehi/Tal1tm2Wehi (Genetic Background: involves: 129S1/Sv * C57BL/6 )

MeSH Terms
  • Animals
  • B-Lymphocytes/cytology
  • Basic Helix-Loop-Helix Transcription Factors
  • Colony-Forming Units Assay
  • DNA-Binding Proteins/genetics
  • Female
  • Gene Expression
  • Gene Targeting
  • Genes, Reporter
  • Genetic Engineering
  • Genetic Vectors
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/metabolism
  • Lac Operon
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Proto-Oncogene Proteins
  • T-Cell Acute Lymphocytic Leukemia Protein 1
  • T-Lymphocytes/cytology
  • Transcription Factors
  • beta-Galactosidase/genetics
  • Blood Vessels/embryology
  • Blood Vessels/physiology
  • Embryonic and Fetal Development/genetics
  • Gene Expression Regulation, Developmental
  • Hematopoietic System/embryology
  • Hematopoietic System/physiology
  • Nerve Tissue/embryology
  • Nerve Tissue/physiology
  • Transcription Factors/genetics
Strain Development
Original mutation in W9.5 ES cell line (129/Sv.C3 genetic background). Chimeric founder mated to C57BL/6. Heterozygous carriers selected and mated to each other. Maintained by mating het x het. Transferred from Dr. Robb to Amgen. Amgen had Charles River Laboratories cryopreserve embryos for them. Embryos from Charles River Laboratories shipped to University of Missouri where two heterozygous males were recovered. Heterozygous males mated to C57BL/6 females. Heterozygous pups from this mating where then mated to each other. Dr. Zhao maintained colony in het x het breeding scheme.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Hematology
  • Research Tools
  • Vasculature System
Donor
Lorraine Robb, Ph.D., Walter and Eliza Hall Institute of Medical Research
Chongbei Zhao, Ph.D., University of Missouri
Primary Reference
  • Elefanty AG, Begley CG, Metcalf D, Barnett L, Kontgen F, Robb L. Characterization of hematopoietic progenitor cells that express the transcription factor SCL, using a lacZ "knock-in" strategy. Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11897-902. (Medline PMID: 9751762)
  • Elefanty AG, Begley CG, Hartley L, Papaevangeliou B, Robb L. SCL expression in the mouse embryo detected with a targeted lacZ reporter gene demonstrates its localization to hematopoietic, vascular, and neural tissues. Blood. 1999 Dec 1;94(11):3754-63. (Medline PMID: 10572089)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030665-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
030665-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.