Strain Detail Sheet

Strain Name    :

STOCK Pirbtm1Stz/Mmucd

Stock Number :

030668-UCD

Other Names   :

PirB-/-, B6;129S4-Lilrb3tm1Stz/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Pirbtm1Stz
Name: targeted mutation 1, Carla J Shatz
Alteration at locus: Knockout
Gene Symbol: Pirb
Name: paired Ig-like receptor B
Chromosome: 7
Alteration at locus: Knockout

Genetic Alterations:
Cre-mediated recombination was used to remove exons 10-13. These exons encode the transmembrane domain and part of the intracellular domain. Western blot failed to detect protein in mutants.

Genotype Determination:

ES Cell Line: J1 derived from 129S4/SvJae

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous: Increased visual cortex plasticity

Heterozygous: Not applicable


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Lilrb3tm1Stz/Lilrb3tm1Stz
        involves: 129S4/SvJae * C57BL/6 * FVB
  • nervous system phenotype
    • nervous system phenotype (MGI Ref ID J:112843)
      • homozygous mice show enhanced cortical ocular-dominance (OD) plasticity at all ages, particularly during the critical period from postnatal days 22 to P31
      • abnormal visual cortex morphology (MGI Ref ID J:112843)
        • after monocular enucleation (ME) from P22-P31, the ipsilateral eye shows greater width of thalamocortical connections in layer 4; width of Arc induction in layer 4 of the binocular zone (BZ) is 22% (P31-P36) to 54% (P19-P25) greater vs wild-type mice and expansion of Arc induction is greater at P100-P110 compared to wild-type (after ME)
        • after monocular deprivation (MD) by eyelid suture from P19-25, width of Arc induction is 17% greater than in wild-type
        • after ME on mice at P25, analysis at P40 shows an 18% wider area of layer 4 receiving inputs from lateral geniculate neurons (thalamocortical connections) in mutants vs wild-type

Strain of Origin: 129S4/SvJae

Strain genetic background: Mixed background of 129S4, C57BL/6 and FVB

Strain Development: The vector was electroporated into 129 J1 ES cells. The resulting mice were crossed with Cre expressing deleter strain B6.FVB-TgN(EIIa-cre)C5379Lmgd expressing Cre recombinase, and mutant PirB gene with deletion of exons 10, 11, 12, and 13.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Immunology and Inflammation
  • Neurobiology

Strain Origin

Donor: Carla Shatz, Ph.D., Stanford University School of Medicine.

Primary Reference:

Syken J, Grandpre T, Kanold PO, Shatz CJ. PirB restricts ocular-dominance plasticity in visual cortex. Science. 2006 Sep 22;313(5794):1795-1800. Epub 2006 Aug 17. (Medline PMID: 16917027)

Colony and Husbandry Information

Special Considerations

Donor added environmental enrichment, such as igloos in breeder cages and nestlets in stock cages.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Brown

Other:

Breeding

MMRRC Breeding System: Backcross or Intra-strain Random Mating

Breeding Scheme(s):

  • Wild-type (C57BL/6J) female x Heterozygous male or reciprocal mating
  • Wild-type female x Heterozygous male of same generation from within the colony or reciprocal mating
  • Homozygous female x Homozygous male of same generation from within the colony or reciprocal mating

Generation:

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: 8 months 8 months

Average litter size: 8

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030668-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030668-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030668-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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