Strain Detail Sheet

Strain Name    :

B6;129P2-Gt(ROSA)26Sortm1(ptxA)Cgh/Mmucd

Stock Number :

030678-UCD

Other Names   :

ROSA26PTX, B6;129P2-Gt(ROSA)26Sortm1(ptxA)Cgh/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Gt(ROSA)26Sortm1(ptxA)Cgh
Name: targeted mutation 1, Shaun R Coughlin
Alteration at locus: Conditional
Gene Symbol: Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Alteration at locus: Conditional

Genetic Alterations:
DNA encoding the ROSA26 Exon 2 splice acceptor followed by a flox PGK Neo polyA X3 flox "Stop" cassette- followed by cDNA for the S1 subunit of Pertussis toxin and a polyA signal was inserted between exon 1 and 2 of the ROSA26 gene.

Genotype Determination:

ES Cell Line: E14 derived from 129P2/OlaHsd

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

The floxed mice do not have a phenotype.  When crossed with an appropriate Cre line, this strain allows lineage-specific ablation of Gi function, providing in vivo models to probe the roles of myriad Gi- coupled receptors in specific cell types of interest in physiology and disease.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Gt(ROSA)26Sortm1(ptxA)Cgh/Gt(ROSA)26Sortm1(ptxA)Cgh
        involves: 129P2/OlaHsd * C57BL/6
  • normal phenotype
    • no abnormal phenotype detected (MGI Ref ID J:130781)
      • mice are indistinguishable from wild-type mice

Strain of Origin: 129P2/OlaHsd

Strain genetic background: B6;129P2

Strain Development: Chimeras generated from targeted E14 ES cels derived from129P2/OlaHsd were mated to C57BL/6 mice to obtain germline transmission of the ROSA26PTX allele. Mice were maintained on a mixed background of 129P2 and C57BL/6.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cancer
  • Cardiovascular
  • Developmental Biology
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor: Shaun Coughlin, M.D., Ph.D., University of California, San Francisco.

Primary Reference:

Regard JB, Kataoka H, Cano DA, Camerer E, Yin L, Zheng YW, Scanlan TS, Hebrok M, Coughlin SR. Probing cell type-specific functions of Gi in vivo identifies GPCR regulators of insulin secretion. J Clin Invest. 2007 Dec;117(12):4034-43. (Medline PMID: 17992256)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Sib-mating

Breeding Scheme(s):

  • Wild-type female x Heterozygous male littermate or reciprocal mating

Generation:

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: 6 months 6 months

Average litter size: 8

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030678-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030678-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030678-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/23/2014