Allele Symbol: Gt(ROSA)26Sortm1(ptxA)Cgh
Name: targeted mutation 1, Shaun R Coughlin
Alteration at locus: Conditional
Gene Symbol: Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Alteration at locus: Conditional
ES Cell Line: E14 derived from 129P2/OlaHsd
The floxed mice do not have a phenotype. When crossed with an appropriate Cre line, this strain allows lineage-specific ablation of Gi function, providing in vivo models to probe the roles of myriad Gi- coupled receptors in specific cell types of interest in physiology and disease.
Strain of Origin: 129P2/OlaHsd
Strain genetic background: B6;129P2
Strain Development: Chimeras generated from targeted E14 ES cels derived from129P2/OlaHsd were mated to C57BL/6 mice to obtain germline transmission of the ROSA26PTX allele. Mice were maintained on a mixed background of 129P2 and C57BL/6.
Suggested Control Mice: Wild-type littermates
Donor: Shaun Coughlin, M.D., Ph.D., University of California, San Francisco.
Regard JB, Kataoka H, Cano DA, Camerer E, Yin L, Zheng YW, Scanlan TS, Hebrok M, Coughlin SR. Probing cell type-specific functions of Gi in vivo identifies GPCR regulators of insulin secretion. J Clin Invest. 2007 Dec;117(12):4034-43. (Medline PMID: 17992256)
Coat color: Black
MMRRC Breeding System: Sib-mating
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||6 months||6 months|
Average litter size: 8
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.