Strain Name:
C57BL/6J-Irf1m1Btlr/Mmucd
Stock Number:
030755-UCD
Citation ID:
RRID:MMRRC_030755-UCD
Other Names:
Endeka
Major Collection:

Strain Information

Irf1M1Btlr
Name: interferon regulatory factor 1; mutation 1, Bruce Beutler
Synonyms: Endeka
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Chemically Induced
Irf1
Name: interferon regulatory factor 1
Synonyms: Irf-1
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 16362
HGNC: HGNC:6116
Homologene: 1658
Genetic Alterations
ENU-induced A to G transition at position 345 of the Irf1 gene, in exon 3 of 10 total exons. The mutation causes an aspartic acid to glycine change at position 47 of the IRF1 protein.
Genotype Determination
Phenotype
Homozygous: The Endeka phenotype was discovered among ENU-induced G3 mutant mice in an in vivo natural killer (NK) cell and CD8+ cytotoxic T lymphocyte (CTL) cytotoxicity screen. G3 mice were immunized with irradiated 5E1 cells (syngeneic class I MHC-deficient cells transformed by human adenovirus type 5 early region 1). One week later, the same mice were injected with three target cell populations: control C57BL/6J cells, NK cell-specific target cells (syngeneic class I MHC-deficient cells), and an antigen- specific CTL target population (C57BL/6J splenocytes externally loaded with the adenovirus E1B protein). Homozygous Endeka mice exhibit a reduced ability to kill class I MHC-deficient cells but kill antigen- specific targets normally, demonstrating impaired NK cell cytotoxicity but normal CD8+ CTL function. Endeka homozygotes display a large reduction of NK1.1+ cells and CD8+ T cells in the blood. There are normal levels of CD4+ T cells, CD19+ B cells, and CD11c+ dendritic cells in the blood of homozygous mice.

Peritoneal macrophages from homozygous Endeka mice produce reduced amounts of type I interferon (IFN) in response to lipopolysaccharide (LPS, TLR4 ligand) and poly I:C (TLR3 ligand). Tumor necrosis factor (TNF)-? production induced by LPS, poly I:C, CpG oligodeoxynucleotides (TLR9 ligand), resiquimod (TLR7 ligand), peptidoglycan (TLR2/6 ligand), macrophage-activating lipopeptide- 2 (MALP-2, TLR2/6 ligand), and Pam3CSK4 (TLR2/1 ligand) is normal in homozygous Endeka macrophages. Homozygous Endeka mice display enhanced susceptibility to infection with 2 x 105 PFU of mouse cytomegalovirus.

Heterozygous: Heterozygous Endeka mice exhibit a reduced ability to kill class I MHC- deficient cells but kill antigen-specific targets normally. The level of killing by heterozygous Endeka NK cells is intermediate between wild type and homozygous Endeka NK cells. Heterozygous mutants display a reduction of NK1.1+ cells intermediate between wild type and homozygotes, while the number of CD8+ T cells is normal. Thus, with respect to the NK cell phenotype, Endeka is semidominant, but with respect to the CD8+ T cell phenotype, Endeka is recessive. There are normal levels of CD4+ T cells, CD19+ B cells, and CD11c+ dendritic cells in the blood of heterozygous mice.

Heterozygous Endeka mice display enhanced susceptibility to infection with 2 x 105 PFU of mouse cytomegalovirus.
Mammalian Phenotype Terms
Allelic Composition: Irf1M1Btlr/Irf1M1Btlr (Genetic Background: C57BL/6J-Irf1M1Btlr )

  • hematopoietic system
    • hematopoietic system phenotype [MP:0005397]
    • decreased NK cell number [MP:0008045]
    • decreased CD8-positive, alpha-beta T cell number [MP:0008079]
    • impaired natural killer cell mediated cytotoxicity [MP:0005070]
  • immune system
    • immune system phenotype [MP:0005387]
    • decreased NK cell number [MP:0008045]
    • decreased CD8-positive, alpha-beta T cell number [MP:0008079]
    • impaired natural killer cell mediated cytotoxicity [MP:0005070]
    • decreased interferon-alpha secretion [MP:0008563]
    • decreased interferon-beta secretion [MP:0008565]
    • increased susceptibility to viral infection [MP:0002418]
Allelic Composition: Irf1M1Btlr/Irf1+ (Genetic Background: C57BL/6J-Irf1M1Btlr )

  • hematopoietic system
    • hematopoietic system phenotype [MP:0005397]
    • decreased NK cell number [MP:0008045]
    • decreased CD8-positive, alpha-beta T cell number [MP:0008079]
    • impaired natural killer cell mediated cytotoxicity [MP:0005070]
  • immune system
    • immune system phenotype [MP:0005387]
    • decreased NK cell number [MP:0008045]
    • decreased CD8-positive, alpha-beta T cell number [MP:0008079]
    • impaired natural killer cell mediated cytotoxicity [MP:0005070]
    • increased susceptibility to viral infection [MP:0002418]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
  • Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Developmental Biology
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
  • Virology
Donor
Bruce Beutler, M.D., The Scripps Research Institute
Primary Reference

Zeng M, Choi JH, Wang K-W, Watthanasurorot A, Murray AR, and Beutler B. Record for endeka. MUTAGENETIX B. Beutler and colleagues, The Scripps Research Institute.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

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MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030755-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.