Strain Detail Sheet

Strain Name    :

FVB/NJ-Tg(Col1a1-tTA)139Niss/Mmucd

Stock Number :

030758-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(Col1a1-tTA)139Niss
Name: transgene insertion 139, Robert Nissenson
Alteration at locus: Transgenic
Promoter: Col1a1
Name: collagen, type I, alpha 1
Alteration at locus: Transgenic
Reporter: tTA
Name: tetracycline-controlled transactivator protein
Alteration at locus: Transgenic

Genetic Alterations:
A plasmid was made by cloning the sequence from the mouse 1-type collagen-I promoter (Col I-2.3) upstream of the tetracycline transactivator (tTA) sequence in plasmid pUGH 15-1. A purified Col I-2.3-tTA DNA fragment was prepared and used to microinject FVB/N oocytes. Injections were carried out at the Transgenic Core Facility of the UCSF-affiliated Gladstone Foundation, according to their standard methods.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: No phenotype until mated to a tTA responsive line

Hemizygous phenotype: No phenotype until mated to a tTA responsive line

Strain of Origin: FVB/NJ

Strain genetic background: FVB/NJ

Strain Development: Original founders were crossed to parental FVB/NJ strain, and maintained as "heterozygotes" for several years. As copy number and site insertion of the transgene was never determined, animals genotyping a positive were considered heterozygous. They were maintained with backcrosses for several years, at which point donor began sib to sib matings to produce a true-breeding line, all matings result in 100% positive offspring and are considered "homozygous". 1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/19/2014