Strain Detail Sheet

Strain Name    :

FVB/N-Tg(Col1a1-tTA)139Niss/Mmmh

Stock Number :

030758-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(Col1a1-tTA)139Niss
Name: transgene insertion 139, Robert Nissenson
Alteration at locus: Transgenic
Promoter: Col1a1
Name: collagen, type I, alpha 1
Alteration at locus: Transgenic
Reporter: tTA
Name: tetracycline-controlled transactivator protein
Alteration at locus: Transgenic

Genetic Alterations:
A plasmid was made by cloning the sequence from the mouse 1-type collagen-I promoter (Col I-2.3) upstream of the tetracycline transactivator (tTA) sequence in plasmid pUGH 15-1. A purified Col I-2.3-tTA DNA fragment was prepared and used to microinject FVB/N oocytes. Injections were carried out at the Transgenic Core Facility of the UCSF-affiliated Gladstone Foundation, according to their standard methods.

Genotype Determination:

ES Cell Line:

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: No phenotype until mated to a tTA responsive line

Hemizygous phenotype: No phenotype until mated to a tTA responsive line

Strain of Origin: FVB/NJ

Strain genetic background: FVB/NJ

Strain Development: Original founders were crossed to parental FVB/NJ strain, and maintained as "heterozygotes" for several years. As copy number and site insertion of the transgene was never determined, animals genotyping a positive were considered heterozygous. They were maintained with backcrosses for several years, at which point donor began sib to sib matings to produce a true-breeding line, all matings result in 100% positive offspring and are considered "homozygous".

The key difference between MMRRC:030758 and MMRRC:030768 is that MMRRC:030758 has tTA driven by a 2.3 kb Col I promoter which is active in mature osteoblasts. By contrast, MMRRC:030768 has tTA driven by a 3.6 kb Col I promoter that is known to be expressed in immature as well as mature osteoblasts. Investigators interested in restricting transgene expression to mature osteoblasts would use MMRRC:030758, whereas those interested in broad expression of the transgene in osteoblast lineage cells would use MMRRC:030768.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Models for Human Disease

Strain Origin

Donor: Robert Nissenson, Ph.D., Veterans Affairs Medical Center

Primary Reference:

  • Peng J, Bencsik M, Louie A, Lu W, Millard S, Nguyen P, Burghardt A, Majumdar S, Wronski TJ, Halloran B, Conklin BR, Nissenson RA. Conditional expression of a Gi-coupled receptor in osteoblasts results in trabecular osteopenia. Endocrinology. 2008 Mar;149(3):1329-37. Epub 2007 Nov 29. (Medline PMID: 18048501)
  • Hsiao EC, Boudignon BM, Chang WC, Bencsik M, Peng J, Nguyen TD, Manalac C, Halloran BP, Conklin BR, Nissenson RA. Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass. Proc Natl Acad Sci U S A. 2008 Jan 29;105(4):1209-14. Epub 2008 Jan 22. (Medline PMID: 18212126)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030758-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
030758-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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