Strain Detail Sheet

Strain Name    :

STOCK Slit3tm1.1Dor/Mmmh

Stock Number :

030759-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Slit3tm1.1Dor
Name: targeted mutation 1.1, David M Ornitz
Alteration at locus: Knockout
Gene Symbol: Slit3
Name: slit homolog 3 (Drosophila)
Chromosome: 11
Alteration at locus: Knockout

Genetic Alterations:
The Slit3 targeting vector was made by introducing a 1-kb deletion encompassing 194 bp of the 3

Genotype Determination:

ES Cell Line: CJ7 derived from 129X1/SvJ

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: small size. Septum transversum diaphramatic hernia where the liver enters the thoracic cavity. Some 40% mice die post P30 of lung compression due to DDH or intestinal obstruction due to herniation of the colon.

Heterozygous phenotype: none


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Slit3tm1.1Dor/Slit3tm1.1Dor
        involves: 129X1/SvJ * C57BL/6J
  • mortality/aging
    • premature death (MGI Ref ID J:83292)
      • phenotype is stated to be identical to that of Slit3tm1Dor homozygotes; however, no data are presented in J:83292
  • growth/size phenotype
    • decreased body weight (MGI Ref ID J:83292)
  • muscle phenotype
    • abnormal diaphragm morphology (MGI Ref ID J:83292)
      • herniated diaphragm (MGI Ref ID J:83292)
      • thin diaphragm muscle (MGI Ref ID J:83292)
  • liver/biliary system phenotype
    • herniated liver (MGI Ref ID J:83292)
  • digestive/alimentary phenotype
    • herniated intestine (MGI Ref ID J:83292)
    • intestinal obstruction (MGI Ref ID J:83292)
  • respiratory system phenotype
    • atelectasis (MGI Ref ID J:83292)
    • lung hemorrhage (MGI Ref ID J:83292)
    • respiratory distress (MGI Ref ID J:83292)
  • cardiovascular system phenotype
    • lung hemorrhage (MGI Ref ID J:83292)

Strain of Origin: 129 es cell into C57BL/6J

Strain Development: Maintained backcross into C57BL6/J before leaving Ornitz.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor: David Ornitz, M.D., Ph.D., Washington University School of Medicine

Primary Reference:

Yuan W, Rao Y, Babiuk RP, Greer JJ, Wu JY, Ornitz DM. A genetic model for a central (septum transversum) congenital diaphragmatic hernia in mice lacking Slit3. Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5217-22. Epub 2003 Apr 17. Erratum in: Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8607. (Medline PMID: 12702769 )

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030759-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030759-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030759-MU-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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