Allele Symbol: Slit3tm1.1Dor
Name: targeted mutation 1.1, David M Ornitz
Alteration at locus: Knockout
Gene Symbol: Slit3
Name: slit homolog 3 (Drosophila)
Alteration at locus: Knockout
ES Cell Line:
Homozygous phenotype: small size. Septum transversum diaphramatic hernia
where the liver enters the thoracic cavity. Some 40% mice die post P30 of
lung compression due to DDH or intestinal obstruction due to herniation of
Heterozygous phenotype: none
Strain of Origin: 129 es cell into C57BL/6J
Strain Development: Maintained backcross into C57BL6/J before leaving Ornitz.
Suggested Control Mice:
Donor: David Ornitz, M.D., Ph.D., Washington University School of Medicine
Yuan W, Rao Y, Babiuk RP, Greer JJ, Wu JY, Ornitz DM. A genetic model for a central (septum transversum) congenital diaphragmatic hernia in mice lacking Slit3. Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5217-22. Epub 2003 Apr 17. Erratum in: Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8607. (Medline PMID: 12702769 )
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.