Strain Detail Sheet

Strain Name    :

LAFT-1/Mmucd

Stock Number :

030769-UCD

Other Names   :

LAFT-1, LAFT-1/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Alteration at locus: Non-mutant

Genetic Alterations:

Genotype Determination:

    ES Cell Line: Not Applicable

    Strain Description [Including phenotype, strain background, strain development and suggested control mice]

    Phenotype

    Homozygous phenotype: The HAFT-1 and LAFT-1 mice were selectively bred for their extreme sensitivity (HAFT-1) and insensitivity (LAFT-1) to the development of acute functional tolerance to ethanol.

    Heterozygous phenotype: Not applicable

    Strain of Origin: HS/Ibg, an 8-way intercross of inbred strains. Strains used in the intercross were A, AK, BALB/c, C3H, C57BL, DBA/2, Is/Br and RIII.

    Strain genetic background: HS/Ibg, an 8-way intercross of inbred strains. Strains used in the intercross were A, AK, BALB/c, C3H, C57BL, DBA/2, Is/Br and RIII.

    Suggested Control Mice:

    • Wildtype littermates

    Research Applications

    • Models for Human Disease
    • Neurobiology
    • Research Tools

    Strain Origin

    Donor: John C. Crabbe, Ph.D., Oregon Health & Science University

    Primary Reference:

    • Erwin VG; Deitrich RA, Genetic selection and characterization of mouse lines for acute functional tolerance to ethanol., J Pharmacol Exp Ther 1996 Dec;279(3):1310-7 (Medline PMID: 8968355)
    • Hu W, Saba L, Kechris K, Bhave SV, Hoffman PL, Tabakoff B. Genomic insights into acute alcohol tolerance. J Pharmacol Exp Ther. 2008 Sep;326(3):792-800. Epub 2008 Jun 11. (Medline PMID: 18550690)
    • Saba L; Bhave SV; Grahame N; Bice P; Lapadat R; Belknap J; Hoffman PL; Tabakoff B, Candidate genes and their regulatory elements: alcohol preference and tolerance., Mamm Genome 2006 Jun;17(6):669-88 (Medline PMID: 16783646 )
    • Hoffman P, Tabakoff B. Gene expression in animals with different acute responses to ethanol. Addict Biol. 2005 Mar;10(1):63-9. (Medline PMID: 15849020)

    Colony and Husbandry Information

    Special Considerations

    None

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    030769-UCD-RESUSLitter recovered from cryo-archive
    $2,022.00 / $4,109.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.
    030769-UCD-EMBRYOCryo-preserved embryos
    $1,038.00 / $2,621.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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