Strain Name:
C57BL/6J-Ifnar1m1Btlr/Mmucd
Stock Number:
030878-UCD
Citation ID:
RRID:MMRRC_030878-UCD
Other Names:
macro-1, C57BL/6J-Ifnar1m1Btlr/Mmcd
Major Collection:

Gene Information

Ifnar1m1Btlr
Name: interferon (alpha and beta) receptor 1; mutation 1, Bruce Beutler
Synonyms: macro-1
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Chemically Induced
Ifnar1
Name: interferon (alpha and beta) receptor 1
Synonyms: Ifrc, Ifar, IFN-alpha/betaR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 15975
HGNC: HGNC:5432
Homologene: 524
Genetic Alterations
An A to C transversion was identified in exon 8 (of 11 total exons) at position 1150 of the Ifnar1 gene. The mutated nucleotide results in a conversion of threonine to proline at residue 341 of the IFNAR1 protein.
Genotype Determination
Phenotype
Homozygous: Peritoneal macrophages from macro-1 mice are highly permissive to MCMV, influenza, RVFV and adenoviral infection. These macrophages produce normal amounts of tumor necrosis factor (TNF)-alpha after Toll-like receptor (TLR) stimulation (TLR Signaling Screen). Preliminary results suggest that mutant macrophages produce lower levels of type I interferons (IFNs) than wild-type macrophages upon adenovirus infection.

Heterozygous: Wild-type.
Mammalian Phenotype Terms
Allelic Composition: Ifnar1m1Btlr/Ifnar1m1Btlr (Genetic Background: C57BL/6J-Ifnar1m1Btlr )

  • immune system
  • hematopoietic system
Strain Development
The macro-1 mutant was generated on a C57BL/6J background by ENU mutagenesis. The strain has been maintained on this background.
Suggested Control Mice
  • Wild-type littermates
  • C57BL/6J
  • Cancer
  • Cell biology
  • Immunology and inflammation
  • Models for human disease
Donor
Bruce Beutler, M.D., The Scripps Research Institute, La Jolla, CA
Primary Reference
Won S, Eidenschenk C, Smart N, Beutler B. Record for macro-1, updated July 23, 2010. MUTAGENETIX (TM), B. Beutler and colleauges, Department of Genetics, The Scripps Research Institute, La Jolla, CA..

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
  • Homozygous female x Homozygous male
  • Heterozygous female x Heterozygous male
Overall Breeding Performance
Good
Average litter size
4-6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
030878-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.