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Availability & Fees Order this Strain
IgA-deficient mice were generated by Igha gene targeting. The targeting vector contains a 5' homologous DNA region located immediately upstream of the I(alpha) exon transcription start site and a 3' homologous DNA region containing the 5' half of the IgA constant region downstream of the EcoRI site. Inserted between the two homologous regions of DNA is a neomycin resistance gene, while an HSV-tk gene is located upstream of the 5' homologous region. Homologous recombination by this targeting vector in ES cells leads to the deletion of the I(alpha) exon, the entire IgA switch region, and the 5' half of the C(alpha) region.
AB2.1 embryonic stem cells (ESCs) derived from 129S7/SvEvBrd-Hprtb-m2 were electroporated with a neomycin selection Igha-targeting construct. Correctly targeted ESCs were injected into C57BL/6 blastocysts followed by implantation into foster mothers. Chimeric males with the greatest degree of chimerism (amount of agouti coat color), were bred to C57BL/6 or 129/Sv female mice. Black agouti offspring were analyzed for transmission of the targeted allele by Southern hybridization of tail DNA. Male and female heterozygous mice were bred to obtain mice homozygous for the targeted deletion. Subsequent backcrossing to C57BL/6J (N8).
Wild-type littermates
Harriman GR, Bogue M, Rogers P, Finegold M, Pacheco S, Bradley A, Zhang Y, Mbawuike IN. Targeted deletion of the IgA constant region in mice leads to IgA deficiency with alterations in expression of other Ig isotypes. J Immunol. 1999 Mar 1;162(5):2521-9. (Medline PMID: 10072491)
Mbawuike IN, Pacheco S, Acuna CL, Switzer KC, Zhang Y, Harriman GR. Mucosal immunity to influenza without IgA: an IgA knockout mouse model. J Immunol. 1999 Mar 1;162(5):2530-7. (Medline PMID: 10072492)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.