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Strain Name:
B6;129S4-Dnmt3btm1Jae/Mmnc
Stock Number:
031043-UNC
Citation ID:
RRID:MMRRC_031043-UNC

Strain Information

Dnmt3btm1Jae
Name: DNA methyltransferase 3B; targeted mutation 1, Rudolf Jaenisch
Synonyms: Dnmt3bL, Dnmt3b2lox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
Dnmt3b
Name: DNA methyltransferase 3B
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Targeted Mutation
NCBI: 13436
HGNC: HGNC:2979
Homologene: 56000
Genetic Alterations
LoxP sites were inserted flanking exons 16-19 of the Dnmt3b gene in V6.5 ES cells. A neomycin resistance gene under the control of the phosphoglycerate kinase promoter and flanked by frt sites was inserted to facilitate selection of integrated clones. The neo cassette was removed by transient lipofection with a flp recombinase-expressing plasmid.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    v6.5 derived from (C57BL/6 x 129S4/SvJae)F1
    Phenotype

    Homozygous and heterozygous: No phenotype

    Cre-excised: Mice are runted and die within a few weeks of birth.

    MeSH Terms
    • Adenoma/genetics
    • Adenoma/pathology
    • Alleles
    • Animals
    • Blotting, Western
    • DNA (Cytosine-5-)-Methyltransferases/genetics
    • DNA (Cytosine-5-)-Methyltransferases/metabolism
    • DNA Methylation
    • DNA, Neoplasm/metabolism
    • Gene Deletion
    • Gene Expression Regulation, Neoplastic
    • Gene Silencing
    • Immunohistochemistry
    • Intestinal Neoplasms/genetics
    • Intestinal Neoplasms/pathology
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Models, Biological
    Strain Development
    targeted in V6.5 ES line, intercrossed or backcrossed to C57BL/6
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Cell Biology
    • Dermatology
    • Developmental Biology
    • Models for Human Disease
    • Reproduction
    • Research Tools
    Donor
    Rudolf Jaenisch, Ph.D., Whitehead Institute
    Primary Reference
    Lin H; Yamada Y; Nguyen S; Linhart H; Jackson-Grusby L; Meissner A; Meletis K; Lo G; Jaenisch R, Suppression of intestinal neoplasia by deletion of Dnmt3b., Mol Cell Biol 2006 Apr;26(8):2976-83 (Medline PMID: 16581773)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    031043-UNC-EMBRYO Cryo-preserved embryos $1,488.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    031043-UNC-SPERM Cryo-preserved spermatozoa $520.00 / Non-Profit Aliquot Approximate quantity3
    031043-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.