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Strain Detail Sheet

Strain Name:
B6;129-Gabpb1tm1Hhx/Mmmh
Stock Number:
031068-MU
Citation ID:
RRID:MMRRC_031068-MU

Strain Information

Gene Details

Gabpb1tm1Hhx
Name: GA repeat binding protein, beta 1; targeted mutation 1, Hai-Hui Xue
Synonyms: GABPbeta1L-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
Gabpb1
Name: GA repeat binding protein, beta 1
Synonyms: E4TF1-53, E4TF1-47, NRF2B2, NRF2B1, E4Tf1B, BABPB2, E4TF1, GABPB1-2, GABPB1-1
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Targeted Mutation
NCBI: 14391
HGNC: HGNC:4074
Homologene: 7723

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
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KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
To specifically eliminate the expression of GABPbeta1L, donor deleted exon 9 in the Gabpb1 gene in the germ line. Donor amplified 4.4-kb and 5.4-kb DNA fragments, which were located upstream and downstream, respectively, of exon 9, from 129 embryonic stem (ES) cell genomic DNA using the Expand Long Template PCR system (Roche). These fragments were cloned upstream and downstream of a neomycin-resistant gene cassette in a gene target vector, pLoxp II. PstI and SacI sites were inserted between the upstream fragment and the neomycin cassette to facilitate the identification of clones with correct homologous recombination after electroporation of the targeting construct into ES cells. The wild-type (WT) allele yields a 7.2-kb PstI fragment when Southern blotted with the upstream probe, whereas the targeted allele was detected as a 5.6-kb fragment . A downstream probe detected a 7.4-kb SacI fragment on the WT allele but a 7.8-kb fragment on the targeted allele.

ES cells with the expected homologous recombination were injected into C57BL/6 blastocysts, and the resulting chimeric mice were mated with C57BL/6 mice to achieve germ line transmission. The heterozygous offspring were then interbred to generate knockout mice.
Genotype Determination
ES Cell Line
Not specified

Strain Description

Phenotype
Homozygous: GABPbeta1L
Mammalian Phenotype Terms
Allelic Composition: Gabpb1tm1Hhx/Gabpb1tm1Hhx (Genetic Background: involves: 129 * C57BL/6 )
MeSH Terms
  • Animals
  • B-Lymphocytes/cytology
  • Embryo Loss
  • Exons/genetics
  • GA-Binding Protein Transcription Factor/metabolism
  • Gene Targeting
  • Mice
  • Promoter Regions, Genetic/genetics
  • Protein Binding
  • Protein Isoforms/metabolism
  • Sequence Deletion
  • T-Lymphocytes/cytology
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor
Warren Leonard, M.D., National Institutes of Health , National Heart, Lung, and Blood Institute (NHLBI)
Primary Reference
Xue HH, Jing X, Bollenbacher-Reilley J, Zhao DM, Haring JS, Yang B, Liu C, Bishop GA, Harty JT, Leonard WJ. Targeting the GA binding protein beta1L isoform does not perturb lymphocyte development and function. Mol Cell Biol. 2008 Jul;28(13):4300-9. Epub 2008 Apr 21. (Medline PMID: 18426908)

Colony and Husbandry Information

Special Considerations

Pathogen free facility

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
031068-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
031068-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.