Strain Detail Sheet

Strain Name    :

MRL.Cg-Tg(Igh-2-12Vh/Ighma)1Shc/Mmnc

Stock Number :

031070-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(Igh-2-12Vh/Ighma)1Shc
Name: transgene insertion 1, Stephen Clarke
Alteration at locus: Transgenic

Genetic Alterations:
Transgenic (Tg) mice were generated using the rearranged V(D)J segment of hybridoma 2-12H cloned upstream of the Igha Cu gene. Purified insert was injected into (C57BL/6 x SJL)F1 blastocysts and then implanted into pseudopregnant mice. Two Tg founders were obtained and further backcrossed to C57BL/6 or C.B17 mice for eight or more generations. Transmission of the transgene was verified by polymerase chain reaction analysis of tail DNA using an oligonucleotide identical to a sequence 470 bp 5' of the 2-12 Vh exon (5'- CAGACTAAGGCCAAATATCAACTGAGAGA-3') and an oligonucleotide complementary to a sequence 3' of Jh4 (5'-CAGGCTCCACCAGACCTCTCTAGT-3').

2-12 MRL/lpr mice were generated by breeding 2-12H-Tg mice with MRL/lpr-Ighb mice and backcrossing to MRL/lpr-Ighb for six to nine generations. The 2-12 H transgene is Igha, making transgene-encoding antibodies distinguishable from endogenous Ighb antibody produced by MRL/lpr mice. The 2-12H MRL mice were generated by backcrossing the 2-12H MRL/lpr mice with Fas intact MRL/Mp+/+ (MRL) mice through 2 generations to eliminate the Faslpr mutation.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype:

Hemizygous phenotype: Increased prelevance of autoimmune disease compare to MRL mice with endogenous Ig secretion.

Strain of Origin: MRL

Strain genetic background: MRL

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Dermatology
  • Immunology and Inflammation
  • Internal/Organ
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor: Barbara Vilen, Ph.D., University of North Carolina at Chapel Hill

Primary Reference:

  • Bloom DD; Davignon JL; Retter MW; Shlomchik MJ; Pisetsky DS; Cohen PL; Eisenberg RA; Clarke SH, V region gene analysis of anti-Sm hybridomas from MRL/Mp-lpr/lpr mice., J Immunol 1993 Feb 15;150(4):1591-610 (Medline PMID: 8432995)
  • Chapoval AI; Nelson H; Thibault C, Anti-CD3 x anti-tumor F(ab')2 bifunctional antibody activates and retargets tumor-infiltrating lymphocytes., J Immunol 1995 Aug 1;155(3):1296-303 (Medline PMID: 7636196)
  • Santulli-Marotto S; Retter MW; Gee R; Mamula MJ; Clarke SH, Autoreactive B cell regulation: peripheral induction of developmental arrest by lupus-associated autoantigens., Immunity 1998 Feb;8(2):209-19 (Medline PMID: 9492002)
  • Halpern MD; Craven SY; Cohen PL; Eisenberg RA, Regulation of anti-Sm autoantibodies by the immunoglobulin heavy chain locus., J Immunol 1993 Dec 15;151(12):7268-72 (Medline PMID: 8258724)

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
031070-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/24/2014