Name: targeted mutation 2, Guoqiang Gu
Alteration at locus: Knock-In
Name: neurogenin 3
Alteration at locus: Knock-In
ES Cell Line: TL1/TL-1 derived from 129S6/SvEvTac
Homozygous phenotype: Mice lack endocrine
islet cells. lack some nerve cells in the neural tubes.
Heterozygous phenotype: Mice have glucose tolerance problem; otherwise healthy and fertile.
Strain of Origin: 129S6/SvEvTac
Strain genetic background: mixed
Strain Development: Knockin ES cells were derived from 129S6/svEVTac strains. Chimera mice were derived and crossed with C56BL/6 animals to obtain germ line transmission. Animals were then crossed with CD1 mice for regular maintenance.
Suggested Control Mice:
Donor: Guoqiang Gu, Ph.D., Vanderbilt University Medical Center
Wang S; Jensen JN; Seymour PA; Hsu W; Dor Y; Sander M; Magnuson MA; Serup P; Gu G, Sustained Neurog3 expression in hormone-expressing islet cells is required for endocrine maturation and function., Proc Natl Acad Sci U S A 2009 Jun 16;106(24):9715-20 (Medline PMID: 19487660)
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.