Strain Name:
B6;129-OoepGt(139A2-3)Cmhd/DeanMmucd
Stock Number:
031076-UCD
Citation ID:
RRID:MMRRC_031076-UCD
Other Names:
Floped tm/tm, B6;129-OoepGt(139A2-3)Cmhd/Mmcd

Strain Information

OoepGt(139A2)Cmhd
Name: oocyte expressed protein; gene trap 139A2, Centre for Modeling Human Disease
Synonyms: Flopedtm, OoepGt(139A2-3)Cmhd
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Gene Trap
Ooep
Name: oocyte expressed protein
Synonyms: 2410146L05Rik, Moep19, Floped
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Gene Trap
NCBI: 67968
Homologene: 12217
Genetic Alterations
Gene-trap vector (Gep-SD5) was inserted into second intron of Floped
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Floped mutation homozygous founders are sterile because the embryos from these mice cannot proceed through embryogenesis

Heterozygous: Heterzygous mice have normal fertility
Mammalian Phenotype Terms
Allelic Composition: OoepGt(139A2)Cmhd/OoepGt(139A2)Cmhd (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

MeSH Terms
  • Animals
  • Antigens/genetics
  • Antigens/metabolism
  • Blastocyst/cytology
  • Blastocyst/physiology
  • Egg Proteins/genetics
  • Egg Proteins/metabolism
  • Embryonic Development/physiology
  • Female
  • Fertilization
  • Humans
  • Infertility, Female/genetics
  • Male
  • Mice
  • Mice, Transgenic
  • Multiprotein Complexes/metabolism
  • Oogenesis/physiology
  • Ovary/cytology
  • Ovary/metabolism
  • Phenotype
  • Pregnancy
  • Protein Binding
  • Proteins/genetics
  • Proteins/metabolism
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Tissue Distribution
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Reproduction
  • Research Tools
Donor
Jurrien Dean, M.D., National Institutes of Health
Primary Reference
Li L, Baibakov B, Dean J. A subcortical maternal complex essential for preimplantation mouse embryogenesis. Dev Cell. 2008 Sep;15(3):416-25. (Medline PMID: 18804437)

Colony and Husbandry Information

Because they are a mix of 129SV and C57BL/6, there is a propensity for obesity which can affect fertility. Donor suggests: keep breeders young and recycle often for long term maintenance. Only male can be homozygous at locus; female must be heterzygous in order to be fertile.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
031076-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
031076-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
031076-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.