Strain Detail Sheet

Strain Name    :

STOCK Tg(Trim2-EGFP)IO71Gsat/Mmucd

Stock Number :

031147-UCD

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(Trim2-EGFP)IO71Gsat
Name: transgene insertion IO71, GENSAT Project at Rockefeller University
Alteration at locus: Transgenic (BAC)
Reporter: EGFP (Jelly Fish)
Name: Enhanced Green Fluorescent Protein
Alteration at locus: Transgenic (BAC)
Promoter: Trim2
Name: tripartite motif-containing 2
Alteration at locus: Transgenic (BAC)

Genetic Alterations:
Genotype modified to contain multiple copies of a modified BAC in which EGFP reporter gene is inserted immediately upstream of the coding sequence of the targeted gene. The BAC address for this line is RP23-15J19.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Site specific expression of green fluorescent protein. In situ histochemistry images of the central nervous system of this mouse can be found at GENSAT. BAC-transgenic images of the central nervous system at various ages of this mouse can be found on the GENSAT project page.

Strain of Origin: FVB/N

Strain genetic background: FVB/N-Crl:CD1(ICR)

Strain Development: Modified BAC containing inserted EGFP upstream of targeted gene injected into pronuclei of FVB/N fertilized oocytes. Hemizygous progeny mated to Crl:CD1(ICR) mice each generation thereafter.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Neurobiology
  • Research Tools

Strain Origin

Donor: Nathaniel Heintz, Ph.D., The Rockefeller University, GENSAT

Primary Reference:

Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, Schambra UB, Nowak NJ, Joyner A, Leblanc G, Hatten ME, Heintz N. A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature. 2003 Oct 30;425(6961):917-25 (Medline PMID: 14586460)

Colony and Husbandry Information

Special Considerations

Hemizygous transgenics are outcrossed to wild-type Crl:CD1(ICR) mates.

In general, GENSAT BAC transgenic mouse lines should be utilized in the hemizygous state. Breeding of the BAC transgene to a homozygous state carries an increased risk of generating an unwanted phenotype for two reasons. First, the transgene inserts at random into the genome. Breeding the transgene to homozygosity results in mice that carry this insertion on both chromosomes, increasing the probability that the insertion will result in an observable phenotype. Second, BAC transgenes may carry passenger genes that are also expressed from the BAC vector. Breeding to homozygosity doubles the number of these genes carried in the transgenic mouse, increasing the possibility that a phenotype will result as a consequence of increased gene dosage. In our experience, observable phenotypes are rare in BAC transgenic mice when carried in the hemizygous state.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: White

Other:

Breeding

MMRRC Breeding System: Outcross to Crl:CD1(ICR)

Breeding Scheme(s):

  • Crl:CD1(ICR) female x Hemizygous male

Generation: N1F2

Overall Breeding Performance: Unknown

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Viability and Fertility:FemaleMale
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Unknown Yes

Age Reproductive Decline: Unknown Unknown

Average litter size: 8

Recommended wean age: 3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
031147-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
031147-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/25/2014