Strain Name:
C57BL/6-Ptprcloc/Mmucd
Stock Number:
031691-UCD
Citation ID:
RRID:MMRRC_031691-UCD
Other Names:
Lochy, C57BL/6JApb-Ptprcloc/Apb/Mmcd, C57BL/6JSfdAnu-Ptprcloc/Apb/AnuApb

Strain Information

Ptprcloc
Name: protein tyrosine phosphatase receptor type C; lochy
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
Ptprc
Name: protein tyrosine phosphatase receptor type C
Synonyms: CD45, T200, Ly-5, Lyt-4, B220
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
Genetic Alterations
The Lochy mutation was mapped to Chromosome 1 and is an ENU-induced T to A transversion at position 91477 of the genomic DNA sequence of Ptprc (Genbank genomic region NC_000067) in intron 17. The mutation creates a splice acceptor site that is used preferentially over the normal splice acceptor site three nucleotides away, and thus results in the insertion of 5 bp (ATCAG) after nucleotide 1791 or 1374 of the mRNA for Ptprc isoform 1 (NM_001111316) or isoform 2 (NM_011210), respectively. The insertion causes a frame shift that introduces 26 aberrant amino acids before a premature stop.
Genotype Determination
Phenotype
Homozygous: Decreased expression of CD45 on CD19? B cells loc/loc mice have normal numbers of B cells Cell intrinsic defect Slightly reduced levels of IgM?IgD?, IgM?IgD? and IgD?CD21? cells Reduced levels of B220loCD19? cells but normal levels of B220?CD19? in bone marrow 3 fold decrease in DP thymocytes 10 fold decrease in CD4 SP thymocytes 5 fold decrease in CD8 SP thymocytes DP thymocytes had 2.5 fold increase in expression of CD4 and CD8, cell intrinsic Co-receptor expression on SP T cells was increased 1.5 fold Dramatic decrease in CD8?CD44lo/med cells TCR? is up-regulated on DP thymocytes and down regulated on mature T cells. CD5 is down-regulated on DP thymocytes and up-regulated on peripheral T cells. Decrease in DP thymocytes is not cell intrinsic Decrease in SP thymocytes is cell intrinsic The decrease of CD69 and CD5 on thymocytes in thymus was cell intrinsic No CD45 protein visually detected by Western blotting Inhibition of DN cells progressing to DP Block preventing DP thymocytes maturing to SP T cells attributed to defective thymic selection Decrease in peripheral T cells Reduction in na ve CD8+ T cells and shift to activated/memory CD8? T cell phenotype Lack of TCR?hi and CD69? cells in thymus-implies defect in TCR signalling for positive selection
Mammalian Phenotype Terms
Allelic Composition: Ptprcloc/Ptprcloc (Genetic Background: C57BL/6JSfdAnu-Ptprcloc/AnuApb )

Strain Development
The Lochy phenotype was identified among ENU-mutagenized G3 mice.
Suggested Control Mice
  • Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell biology
  • Immunology and inflammation
Donor
Chris Goodnow, BVSc, PhD, The Australian National University, Canberra, Australia

Colony and Husbandry Information

This strain is also reposited as Lochy at the NHMRC Australian PhenomeBank (APB).

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Other
Homozygotes lack T cells , but do well in a clean environment.
MMRRC Breeding System
Backcross
Generation
N3F7
Overall Breeding Performance
Excellent
Average litter size
6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
031691-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.