Strain Name    : C57BL/6J-Slfn2m1Btlr/Mmucd

Stock Number : 031705-UCD

Other Names   : elektra, C57BL/6J-Slfn2m1Btlr/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Slfn2m1Btlr
Name: mutation 1, Bruce Beutler
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Slfn2
Name: schlafen 2
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations:
An ENU-induced T to A transversion occurred at position 586 of the Slfn2 gene (Genbank Accession NM_011408). The recessive mutation results in an isoleucine to asparagine change at position 135 of the Schlafen2 protein.

Genotype Determination:

ES Cell Line: Not applicable

Additional mutations found on this strain:

AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02141:Slfn2 APN 11 83069844 missense probably damaging 0.96
IGL02407:Slfn2 APN 11 83069576 missense probably benign 0.00
R2270:Slfn2 UTSW 11 83069935 missense probably damaging 0.99
R2392:Slfn2 UTSW 11 83065328 missense possibly damaging 0.91
R3436:Slfn2 UTSW 11 83069564 missense probably benign 0.05
R3437:Slfn2 UTSW 11 83069564 missense probably benign 0.05

Phenotype

Homozygous phenotype: Homozygous mice show increased susceptibility to infection by mouse cytomegalovirus (MCMV), listeria monocytogenes, and lymphocytic choriomeningitis virus (LCMV). Natural killer (NK) cell numbers are significantly increased in the thymus, spleen and liver of homozygotes, while low T cell (both CD4 and CD8) numbers are seen in the spleen and lymph node, and low CD8 and slightly reduced CD4 T cell numbers are seen in the blood. When transferred to an irradiated congenic host, homozygous elektra T cells fail to repopulate the spleen, however, elektra B cells reconstitute the spleen normally. Bcl-2 expression is specifically reduced in homozygous elektra CD8+CD44(high) T cells, but is normal in CD8+CD44(low) T cells.

Heterozygous phenotype: Wild-type.

Mammalian Phenotype Terms:
Allelic Composition: Slfn2m1Btlr/Slfn2m1Btlr (Genetic Background: C57BL/6J-Slfn2m1Btlr )

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: The original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain the strain were on a C57BL/6J background only.

Suggested Control Mice:

  • Wild-type littermates
  • C57BL/6J

  • Apoptosis
  • Immunology and inflammation
  • Research tools

Donor: Bruce Beutler, M.D., The Scripps Research Institute, La Jolla, CA

Primary Reference:

  • Berger M, Moresco E, Beutler B. Record for “elektra”, updated March 15, 2010. MUTAGENETIX (TM), B. Beutler and colleagues, Department of Genetics, The Scripps Research Institute, La Jolla, CA.
  • Berger M; Krebs P; Crozat K; Li X; Croker BA; Siggs OM; Popkin D; Du X; Lawson BR; Theofilopoulos AN; Xia Y; Khovananth K; Y Moresco EM; Satoh T; Takeuchi O; Akira S; Beutler B, An Slfn2 mutation causes lymphoid and myeloid immunodeficiency due to loss of immune cell quiescence., Nat Immunol 2010 Apr;11(4):335-43 (Medline PMID: 20190759)
  • Berger M; Krebs P; Crozat K; Li X; Croker BA; Siggs OM; Popkin D; Du X; Lawson BR; Theofilopoulos AN; Xia Y; Khovananth K; Y Moresco EM; Satoh T; Takeuchi O; Akira S; Beutler B, An Slfn2 mutation causes lymphoid and myeloid immunodeficiency due to loss of immune cell quiescence., Nat Immunol 2010 Apr;11(4):335-43 (Medline PMID: 20190759)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: Black

Other:


MMRRC Breeding System: Random intra-strain mating

Breeding Scheme(s):

  • Homozygous female x Homozygous male
  • Heterozygous female x Heterozygous male

Generation:

Overall Breeding Performance: Good

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: Unknown Unknown

Average litter size: 4-6

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
031705-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
031705-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
031705-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.