Strain Detail Sheet

Strain Name    :

B6.129S1-Mei1m1Jcs/Mmnc

Stock Number :

031721-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mei1m1Jcs
Name: meiosis defective 1
Alteration at locus: Chemically Induced
Gene Symbol: Mei1
Name: meiosis defective 1
Chromosome: 15
Alteration at locus: Chemically Induced

Genetic Alterations:
The molecular defect is an A-to-T transversion at the AG splice acceptor sequence of intron 11. Two aberrantly spliced transcripts are expressed from this allele, both resulting in a frameshift of the wild-type sequence and a predicted truncated protein.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Male and female infertility due to meiotic arrest in the zygotene stage of meiosis I. These mice are defective in formation of genetically-programmed double strand breaks, which blocks recombination and syapsis from occurring properly.

Heterozygous phenotype:


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Mei1m1Jcs/Mei1m1Jcs
        involves: 129S1/Sv * C57BL/6J
  • reproductive system phenotype
    • abnormal female meiosis (MGI Ref ID J:60747)
      • meiotic defects
    • arrest of male meiosis (MGI Ref ID J:60747)
      • spermatocytes arrest at zygotene stage of meiosis I
    • azoospermia (MGI Ref ID J:60747)
      • absent sperm, absence of post-meiotic cells
    • female infertility (MGI Ref ID J:60747)
    • male infertility (MGI Ref ID J:60747)
    • small testis (MGI Ref ID J:60747)
  • endocrine/exocrine gland phenotype
    • small testis (MGI Ref ID J:60747)

Strain of Origin: 129S1/SvImJ

Strain genetic background: C57BL/6J

Strain Development: See MGI:2176973. The mutant line was derived from EMS mutagenesis of CJ7 ES cells. Over the years, donor backcrossed to C57BL/6J. At steps along the way, intercrosses were conducted, but derivatives have been crossed to B6 for the current stocks.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6.129S1-Mei1m1Jcs/Mmnc MMRRC:031721-UNC C57BL/6J 129S1/SvImJ 2572 0 9 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Reproduction

Strain Origin

Donor: John Schimenti, Ph.D., Cornell University Coll. Veterinary Medicine

Primary Reference:

  • Libby BJ; Reinholdt LG; Schimenti JC, Positional cloning and characterization of Mei1, a vertebrate-specific gene required for normal meiotic chromosome synapsis in mice., Proc Natl Acad Sci U S A 2003 Dec 23;100(26):15706-11 (Medline PMID: 14668445)
  • Libby BJ; De La Fuente R; O'Brien MJ; Wigglesworth K; Cobb J; Inselman A; Eaker S; Handel MA; Eppig JJ; Schimenti JC, The mouse meiotic mutation mei1 disrupts chromosome synapsis with sexually dimorphic consequences for meiotic progression., Dev Biol 2002 Feb 15;242(2):174-87 (Medline PMID: 11820814)
  • Munroe RJ, Bergstrom RA, Zheng QY, Libby B, Smith R, John SW, Schimenti KJ, Browning VL, Schimenti JC. Mouse mutants from chemically mutagenized embryonic stem cells. Nat Genet. 2000 Mar;24(3):318-21. (Medline PMID: 10700192 )

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
031721-UNC-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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