Strain Name:
B6.129X1-Kcnj3tm1Kwn/Mmucd
Stock Number:
031758-UCD
Citation ID:
RRID:MMRRC_031758-UCD
Other Names:
KCNJ3null, Kir3.1null , GIRK1-/- , B6.129X1-Kcnj3tm1Kwn/Mmcd

Strain Information

Kcnj3tm1Kwn
Name: potassium inwardly-rectifying channel, subfamily J, member 3; targeted mutation 1, Kevin D Wickman
Synonyms: GIRK1 KO, Kir3.1 knock-out
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
Kcnj3
Name: potassium inwardly-rectifying channel, subfamily J, member 3
Synonyms: Kcnf3, GIRK1, Kir3.1
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Knockout
NCBI: 16519
HGNC: HGNC:6264
Homologene: 1687
Genetic Alterations
The Kcnj3 exon 3 was deleted by homologous and cre mediated recombination in ES cells. The deleted sequence encoded the pore and transmembrane domains, the entire amino terminus, and the initiator methionine. Normal protein is undetected by Western blot analysis of atrial tissue obtained from homozygous mutant mice.
ES Cell Line
Unspecified
Phenotype
Kcnj3 (GIRK1) is a widely expressed subunit in the CNS. Currents carried by GIRK channels are a major component of slow inhibitory postsynaptic currents seen in many neuron populations.

Homozygous: null mice prone to seizures around 2-6 months of age.

Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Kcnj3tm1Kwn/Kcnj3tm1Kwn (Genetic Background: involves: 129X1/SvJ )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • G Protein-Coupled Inwardly-Rectifying Potassium Channels
  • Heart Atria/metabolism
  • Ion Channel Gating/physiology
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Potassium Channels/genetics
  • Potassium Channels/physiology
  • Potassium Channels, Inwardly Rectifying
  • Receptors, Muscarinic/physiology
Strain Development
Suitable 5′ and 3′ Kir3.1homology arms were subcloned into a pBluescript-based plasmid containing a neomycin resistance gene (NEO) driven by the mouse PGK promoter. The NEO cassette was flanked by Cre recombinase recognition sites (loxP sites). The diphtheria toxin (DTA) gene driven by the thymidine kinase promoter was included in the construct as a negative selection element to enrich for homologous recombinants. 129Sv/J embryonic stem cells at passage 11 (Genome Systems, St. Louis, MO) were transfected with the linearized Kir3.1 targeting vector as described and 692 colonies surviving G418 selection (200 μg/ml active constituent for 10 days) were picked, amplified, and screened by PCR and Southern blotting for the appropriate homologous recombination event. A single embryonic stem cell clone harboring the targeted allele was amplified and transfected with a plasmid containing the Cre recombinase cDNA driven by the herpes simplex virus thymidine kinase promoter. Two lines were identified that harbored the null mutant Kir3.1 allele, and both lines were used successfully to generate germline-transmitting chimeric mice. All mice in this study were genotyped either by Southern blotting or by PCR using tail DNA. The donating investigator reports that this mutant mouse line was backcrossed to C57BL/6 at least 19 times.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Diabetes/Obesity
  • Internal/Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Research Tools
  • Sensorineural
Donor
Kevin Wickman, Ph.D., University of Minnesota
Primary Reference
Bettahi I, Marker CL, Roman MI, Wickman K. Contribution of the Kir3.1 subunit to the muscarinic-gated atrial potassium channel IKACh. J Biol Chem. 2002 Dec 13;277(50):48282-8. Epub 2002 Oct 8. (Medline PMID: 12374786)

Colony and Husbandry Information

Homozygous null mice will breed but this is typically not done due to seizure propensity.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
031758-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
031758-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
031758-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.