Strain Name:
B6.129X1-Kcnj9tm1Kwn/Mmucd
Stock Number:
031759-UCD
Citation ID:
RRID:MMRRC_031759-UCD
Other Names:
KCNJ9null, Kir3.3null , GIRK3-/- , B6.129X1-Kcnj9tm1Kwn/Mmcd

Strain Information

Kcnj9tm1Kwn
Name: potassium inwardly-rectifying channel, subfamily J, member 9; targeted mutation 1, Kevin Wickman
Synonyms: GIRK3 KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knockout
Kcnj9
Name: potassium inwardly-rectifying channel, subfamily J, member 9
Synonyms: Girk3, 1700085N21Rik, Kir3.3
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 16524
HGNC: HGNC:6270
Homologene: 37989
Genetic Alterations
A single loxP site remained in place of Kcnj9 exon 3 after the cre-mediated excision of a floxed region containing exon 3 and an inserted neo cassette. The deleted exon encoded the majority of the protein including the pore- and membrane-spanning domains and the entire N-terminal region and a substantial portion of the C-terminal region. Protein was undetected by Western blot analysis of homozygous mutant brain tissue.

ES Cell Line
Unspecified.
Phenotype

Drug dependence and analgesia currents carried by GIRK channels are a major component of
slow inhibitory postsynaptic currents seen in many neuron populations.

Homozygous: no distinguishable phenotype

Heterozygous: no distinguishable phenotype

Mammalian Phenotype Terms
Allelic Composition: Kcnj9tm1Kwn/Kcnj9tm1Kwn (Genetic Background: involves: 129X1/SvJ )

MeSH Terms
  • Animals
  • Barium/pharmacology
  • Calcium/metabolism
  • Enkephalin, Methionine/pharmacology
  • Female
  • G Protein-Coupled Inwardly-Rectifying Potassium Channels
  • GTP-Binding Proteins/metabolism
  • Heterozygote
  • Homozygote
  • Ion Channel Gating/drug effects
  • Ion Channel Gating/physiology
  • Locus Coeruleus/cytology
  • Locus Coeruleus/drug effects
  • Locus Coeruleus/physiology
  • Male
  • Membrane Potentials/drug effects
  • Membrane Potentials/physiology
  • Mice
  • Mice, Knockout
  • Narcotics/pharmacology
  • Neural Inhibition/drug effects
  • Neural Inhibition/physiology
  • Neurons/drug effects
  • Neurons/physiology
  • Patch-Clamp Techniques
  • Potassium Channel Blockers/pharmacology
  • Potassium Channels/deficiency
  • Potassium Channels/genetics
  • Potassium Channels/metabolism
  • Potassium Channels, Inwardly Rectifying/antagonists & inhibitors
  • Potassium Channels, Inwardly Rectifying/deficiency
  • Potassium Channels, Inwardly Rectifying/genetics
  • Potassium Channels, Inwardly Rectifying/metabolism
  • Protein Subunits
Strain Development
A Cre recombinase-based targeting strategy was designed with the goal of obtaining a parental mouse embryonic stem (ES) cell line that could be further modified to yield ES cell clonal
derivatives harboring either a Kir3.3 gene KO or a “floxed” version of the mouse Kir3.3 gene.
A single loxP site remained in place of exon 3 after the cre-mediated excision of a floxed region containing exon 3 and an inserted neo cassette. The deleted exon encoded the majority of the protein including the pore- and membrane-spanning domains and the entire N-terminal region and a substantial portion of the C-terminal region. Protein was undetected by Western blot analysis of homozygous mutant brain tissue.
KO mice were backcrossed to C57BL/6 mice for at least 23 generations.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Metabolism-
  • Neurobiology
  • Research Tools
  • Sensorineural
Donor
Kevin Wickman, Ph.D., University of Minnesota
Primary Reference
Torrecilla M, Marker CL, Cintora SC, Stoffel M, Williams JT, Wickman K. G-protein-gated potassium channels containing Kir3.2 and Kir3.3 subunits mediate the acute inhibitory effects of opioids on locus ceruleus neurons. J Neurosci. 2002 Jun 1;22(11):4328-34. (Medline PMID: 12040038)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
031759-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
031759-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
031759-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.