Strain Name:
B6.Cg-Tg(Klk8-tTA)SMmay/MullMmmh
Stock Number:
031779-MU
Citation ID:
RRID:MMRRC_031779-MU
Major Collection:

Gene Information

tTA
Name: tetracycline-controlled transactivator protein
Type: Gene
Species: Multi-species
Klk8
Name: kallikrein related-peptidase 8
Synonyms: BSP1, Nrpn, Prss19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259277
HGNC: HGNC:6369
Homologene: 21410
Tg(Klk8-tTA)SMmay
Name: transgene insertion S, Mark Mayford
Synonyms: Prss19-tTA line 1, Nop-tTA, tTA-EC, Neuropsin-htTa, Prss19-tTA1, TgEC-tTA
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genotype Determination
Phenotype
These neuropsin-tTA BAC transgenic line S mice (also called Prss19-tTA line 1, Prss19-tTA1, tTA-EC, Nop-tTA, Neuropsin-htTa) are viable and fertile, with expression of a human codon-optimized tetracycline-regulated transactivator protein (tTA or htTA) under control of the mouse kallikrein related-peptidase 8 (Klk8; also called Prss19 or neuropsin) promoter/enhancer regions on the BAC transgene. For neuropsin-tTA BAC transgenic mice derived from founder line S, tTA activity is primarily observed in specific regions of the medial temporal lobe of the brain, including the superficial layers of the medial entorhinal cortex (MEC) as well as the pre- and parasubiculum (Pr-PaS). Very few neurons exhibit tTA activity in lateral entorhinal cortex, and no tTA activity is reported in the hippocampus. When mated to a second strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene can be blocked by administration of the tetracycline analog, doxycycline (dox). These neuropsin-tTA BAC transgenic line S mice are a "Tet-Off" tool that allow the dox-inducible expression of genes primarily in the superficial entorhinal cortex. Because the superficial entorhinal cortex is located in the temporal lobe of the cerebral cortex as the main input region to the hippocampus and functions as an important memory center, these mice may be useful in studying spatial learning and memory.
Strain Development
These neuropsin-tTA BAC transgenic line S mice (also called Prss19-tTA line 1, Prss19-tTA1, tTA-EC, Nop-tTA, Neuropsin-htTa) were designed by Dr. Mark Mayford (Columbia University, New York). An ~120 kb mouse bacterial artificial chromosome (BAC) containing the entire mouse kallikrein related-peptidase 8 (Klk8; also called Prss19 or neuropsin) locus, and ~50 kb of upstream and ~70 kb of downstream sequences, was modified by targeting a human codon-optimized tetracycline-regulated transactivator gene (tTA or htTA or "Tet-Off") into the first exon upstream of the Prss19 translation initiation site. The resulting modified BAC was microinjected into (C57BL/6;DBA)F2 embryos. Founder mice were bred to C57BL/6J inbred mice to establish founder line S. These neuropsin-tTA BAC transgenic line S mice were obtained by Dr. Ulrich Mueller (The Scripps Research Institute) and subsequently maintained by breeding with C57BL/6J inbred mice (total of more than six generations) prior to sending to The Jackson Laboratory. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.
Suggested Control Mice
  • Wildtype littermates
Donor
Ulrich Mueller, Ph.D., The Scripps Research Institute
Primary Reference
Additional information regarding this strain can be found at NIH Neuroscience Blueprint Cre Driver Network.

Colony and Husbandry Information

When maintained as a live colony, hemizygous mice should be bred with C57BL/6J inbred wildtype mice.

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@missouri.edu for more details.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
031779-MU-HET-F
031779-MU-HET-M
031779-MU-WT-F
031779-MU-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
$290.00 / Non-Profit Per Mouse The mmrrc@missouri.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
031779-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.