Strain Name:
B6.129-Htr2ctm2Knk/Mmmh
Stock Number:
031782-MU
Citation ID:
RRID:MMRRC_031782-MU

Strain Information

Htr2ctm2Knk
Name: 5-hydroxytryptamine (serotonin) receptor 2C; targeted mutation 2, Kazuko Nishikura
Synonyms: 5-HT2CR-INI
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knock-In
Htr2c
Name: 5-hydroxytryptamine (serotonin) receptor 2C
Synonyms: 5HT1c, 5-HT2C receptor, Htr1c, SR1, 5-HT2cR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knock-In
NCBI: 15560
HGNC: HGNC:5295
Homologene: 20242
Genetic Alterations
RNA editing that converts adenosine to inosine replaces the gene-encoded Ile, Asn, and Ile (INI) of serotonin (5-hydroxytryptamine, 5-HT) receptor 2C (5-HT2CR) with Val, Gly, and Val (VGV). Up to 24 different 5-HT2CR isoforms are detected in different brain regions. To elucidate the physiological significance of 5-HT2CR mRNA editing, Donor derived mutant mouse lines harboring a knock- in INI or VGV allele, resulting in sole expression of one of two extremely different editing isoforms 5-HT2CR-INI (editing blocked) or - VGV (fully edited).

Inosine is interpreted as guanosine by the translational machinery. In the 5-HT2CR-VGV knock-in vector, five A-G mutations at sites A-E were introduced, while the editing complementary sequence in intron 5 (160 bp) was deleted in the 5-HT2CR-INI targeting vector. A silent Xba 1 site created upstream of the editing sites in exon 5 facilitated screening of the mutated allele in ES cells and knock- in mice. ES cell clones selected for G418 resistance and ganciclovir sensitivity were screened by PCR using a set of primers: the intron 4 primer, 5
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Mice display behavioral changes (more nervousness).

Hemizygous: Undetermined.
Mammalian Phenotype Terms
Allelic Composition: Htr2ctm2Knk/Htr2ctm2Knk (Genetic Background: either: B6.Cg-Htr2ctm2Knk or C.Cg-Htr2ctm2Knk )

Allelic Composition: Htr2ctm1Knk/Htr2ctm2Knk (Genetic Background: B6.Cg-Htr2ctm1Knk/Htr2ctm2Knk )

MeSH Terms
  • Adipose Tissue/metabolism
  • Amino Acid Sequence/genetics
  • Animals
  • Energy Metabolism/genetics
  • Female
  • Gene Knock-In Techniques
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mutation/genetics
  • Protein Isoforms/genetics
  • RNA Editing/genetics
  • RNA, Messenger/genetics
  • Receptor, Melanocortin, Type 4/genetics
  • Receptor, Melanocortin, Type 4/metabolism
  • Receptor, Serotonin, 5-HT2C/genetics
  • Serotonin/metabolism
  • Signal Transduction/genetics
  • Sympathetic Nervous System/physiopathology
  • Synaptic Transmission/genetics
Strain Development
The founder male mice were backcrossed to wt C57BL/6 female mice. More than 10 backcrossing were done.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Diabetes/Obesity
  • Metabolism
  • Models for Human Disease
  • Neurobiology
Donor
Kazuko Nishikura, Ph.D., The Wistar Institute
Primary Reference
Kawahara Y, Grimberg A, Teegarden S, Mombereau C, Liu S, Bale TL, Blendy JA, Nishikura K. Dysregulated editing of serotonin 2C receptor mRNAs results in energy dissipation and loss of fat mass. Neurosci. 2008 Nov 26;28(48):12834-44. (Medline PMID: 19036977)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
031782-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
031782-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.