Strain Name:
STOCK E2f5tm1Dli/Mmucd
Stock Number:
031809-UCD
Citation ID:
RRID:MMRRC_031809-UCD
Other Names:
E2F-5 mix, STOCK E2f5tm1Dli/Mmcd

Strain Information

E2f5tm1Dli
Name: E2F transcription factor 5; targeted mutation 1, David M Livingston
Synonyms: E2F-5-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
E2f5
Name: E2F transcription factor 5
Synonyms: E2F-5
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Knockout
NCBI: 13559
HGNC: HGNC:3119
Homologene: 1472
Genetic Alterations
Replacement of the two exons encoding the carboxy-termainl half of the DNA binding and leucine zipper domains
ES Cell Line
CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Phenotype
Homozygous: Kos develop hydrocephaly and die from it.

Heterozygous: Viable and used to maintain the strain
Mammalian Phenotype Terms
Allelic Composition: E2f5tm1Dli/E2f5tm1Dli (Genetic Background: either: (involves: 129S1/Sv * BALB/c) or (involves: 129S1/Sv * C57BL/6) )

MeSH Terms
  • Alleles
  • Animals
  • Cell Division
  • Choroid Plexus/metabolism
  • Choroid Plexus/physiology
  • E2F5 Transcription Factor
  • Gene Deletion
  • Gene Targeting
  • Humans
  • Hydrocephalus/metabolism
  • Mice
  • Mice, Knockout
  • Transcription Factors/biosynthesis
  • Transcription Factors/genetics
  • Transcription Factors/physiology
Strain Development
Mice at Peter mac were intracrossed within the colony but frozen embryos are made up of E2F5 +/- B6129S6SvTac BaLB/c males mated to females B6 129 S4SvJae 129SvTac so embryos a mix of B6 129S4Svjae129S6SvTac BaLB/c
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Developmental Biology
Donor
David M Livingston M.D., Ph.D., Dana Farber Cancer Institute
Primary Reference
Lindeman GJ; Dagnino L; Gaubatz S; Xu Y; Bronson RT; Warren HB; Livingston DM, A specific, nonproliferative role for E2F-5 in choroid plexus function revealed by gene targeting., Genes Dev 1998 Apr 15;12(8):1092-8 (Medline PMID: 9553039)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
031809-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
031809-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
031809-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.