Allele Symbol: Rgs9tm1Citb
Name: targeted mutation 1, California Institute of Technology - Biology
Alteration at locus: Knockout
Gene Symbol: Rgs9
Name: regulator of G-protein signaling 9
Alteration at locus: Knockout
ES Cell Line: CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Homozygous phenotype: Mice
have delayed recovery of photoreceptor light responses. Also, they show
heightened locomotor and rewarding responses to cocaine and related
Heterozygous phenotype: No obvious phenotypic expression.
Strain of Origin: 129X1/SvJ
Strain genetic background: C57BL/6J
Strain Development: The heterozygous mice were bred to
C57BL/6J for eight generations at U. of Minnesota.
Strain Genetic Background Validation:
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
|MMRRC Strain||Reference Strains||Genomic makeup (Mb)|
|Strain Name||MMRRC ID #||Reference 1||Reference 2||Ref 1||Ref 2||Het||Unknown|
Suggested Control Mice:
Donor: Ching-Kang Jason Chen, Ph.D., Virginia Commonwealth University
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.