Strain Detail Sheet

Strain Name    :

B6.129S1-Rgs9tm1Citb/Mmnc

Stock Number :

031829-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Rgs9tm1Citb
Name: targeted mutation 1, California Institute of Technology - Biology
Alteration at locus: Knockout
Gene Symbol: Rgs9
Name: regulator of G-protein signaling 9
Chromosome: 11
Alteration at locus: Knockout

Genetic Alterations:
A new cassette replaced a 4 Kb fragment containing exons 2 to 4.

Genotype Determination:

ES Cell Line: CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Mice have delayed recovery of photoreceptor light responses. Also, they show heightened locomotor and rewarding responses to cocaine and related psychostimulants.

Heterozygous phenotype: No obvious phenotypic expression.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Rgs9tm1Citb/Rgs9tm1Citb
        either: 129S1/Sv-Rgs9<tm1Citb> or (involves: 129S1/Sv * C57BL/6)
  • vision/eye phenotype
    • vision/eye phenotype (MGI Ref ID J:60188)
      • homozygotes displayed normal retinal morphology up to 8 months of age
      • mutant retinas contained normal amounts of other photoreceptor proteins
      • abnormal eye physiology (MGI Ref ID J:60188)
        • mutant photoreceptors displayed abnormally retarded recovery of their light responses and slowed rates of GTP hydrolysis by transducin relative to wild-type controls
        • the mutation had no effect on the activation of the G-protein cascade, but significantly prolonged its deactivation and interfered with the speeding of incremental dim flashes during light adaptation
        • abnormal rod electrophysiology (MGI Ref ID J:60188)
          • mutant rods exhibited extremely slowed photoresponses, recovering from moderate intensity flashes with a dominant time constant (9 s) that is 45-fold longer than that in wild-type mice (0.2 s)

Strain of Origin: 129X1/SvJ

Strain genetic background: C57BL/6J

Strain Development: The heterozygous mice were bred to C57BL/6J for eight generations at U. of Minnesota.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6.129S1-Rgs9tm1Citb/Mmnc MMRRC:031829-UNC C57BL/6J 129S1/SvImJ 2568 0 14 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Models for Human Disease
  • Neurobiology
  • Sensorineural

Strain Origin

Donor: Ching-Kang Jason Chen, Ph.D., Virginia Commonwealth University

Primary Reference:

  • Chen CK, Burns ME, He W, Wensel TG, Baylor DA, Simon MI. Slowed recovery of rod photoresponse in mice lacking the GTPase accelerating protein RGS9-1. Nature. 2000 Feb 3;403(6769):557-60. (Medline PMID: 10676965)
  • Rahman Z, Schwarz J, Gold SJ, Zachariou V, Wein MN, Choi KH, Kovoor A, Chen CK, DiLeone RJ, Schwarz SC, Selley DE, Sim-Selley LJ, Barrot M, Luedtke RR, Self D, Neve RL, Lester HA, Simon MI, Nestler EJ. RGS9 modulates dopamine signaling in the basal ganglia. Neuron. 2003 Jun 19;38(6):941-52. (Medline PMID: 12818179 )

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
031829-UNC-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
031829-UNC-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
031829-UNC-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 04/23/2014