Strain Detail Sheet

Strain Name    :

B6.129S2-Pak1tm1Cher/Mmnc

Stock Number :

031838-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Pak1tm1Cher
Name: targeted mutation 1, Jonathan Chernoff
Alteration at locus: Knockout
Gene Symbol: Pak1
Name: p21 protein (Cdc42/Rac)-activated kinase 1
Chromosome: 7
Alteration at locus: Knockout

Genetic Alterations:
neo cassette inserted into exon 4, eliminating exon 5 and disrupted p21-binding domain and causing premature truncation of protein

Genotype Determination:

ES Cell Line: D3

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Defects in mast cell function (motility, degranulation, and cytokine production) and macrophage function (spreading); defects in long-term potentiation; defects in viral infectivity

Heterozygous phenotype: none


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Pak1tm1Cher/Pak1tm1Cher
        B6.129S2-Pak1<tm1Cher>
  • immune system phenotype
    • abnormal mast cell physiology (MGI Ref ID J:142439)
      • bone marrow derived mast cells proliferate at 60% the rate as controls when cultured for three days with SCF
      • these mast cells also have a third slower migration rate towards SCF in transwell assays than controls
      • in vivo, less mast cells migrate to the skin when SCF is administered than in controls
      • decreased mast cell degranulation (MGI Ref ID J:145444)
        • bone marrow derived mast cells have a 3-fold reduction in granule release upon Fc-epsilionRI stimulation compared to controls
        • calcium flux from the extracellular environment is significantly reduced after receptor stimulation compared to controls
        • when passive cutaneous anaphylaxis (PCA) is induced in the ear of mice, the mast cells fail to respond
        • similar results are observed when mutant mast cells are transferred into mast-cell deficient mice and PCA is induced

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: Initial KO mouse (129/Bl6) backcrossed >10 generations to C57BL/6.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6.129S2-Pak1tm1Cher MMRRC:031838-UNC C57BL/6J 129S1/SvImJ 2533 0 49 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cancer
  • Immunology and Inflammation
  • Neurobiology
  • Research Tools
  • Virology

Strain Origin

Donor: Jonathan Chernoff , Ph.D., Fox Chase Cancer Center

Primary Reference:

  • Allen JD, Jaffer ZM, Park SJ, Burgin S, Hofmann C, Sells MA, Chen S, Derr-Yellin E, Michels EG, McDaniel A, Bessler WK, Ingram DA, Atkinson SJ, Travers JB, Chernoff J, Clapp DW. p21-activated kinase regulates mast cell degranulation via effects on calcium mobilization and cytoskeletal dynamics. Blood. 2009 Mar 19;113(12):2695-705. Epub 2009 Jan 5. (Medline PMID: 19124833)
  • McDaniel AS, Allen JD, Park SJ, Jaffer ZM, Michels EG, Burgin SJ, Chen S, Bessler WK, Hofmann C, Ingram DA, Chernoff J, Clapp DW. Pak1 regulates multiple c-Kit mediated Ras-MAPK gain-in-function phenotypes in Nf1+/- mast cells. Blood. 2008 Dec 1;112(12):4646-54. Epub 2008 Sep 2. (Medline PMID: 18768391 )
  • Hofmann C, Shepelev M, Chernoff J. The genetics of Pak. J Cell Sci. 2004 Sep 1;117(Pt 19):4343-54. PMID: 15331659 (Medline PMID: 15331659)

Colony and Husbandry Information

Special Considerations

Mice are black in coat color, with the possibility of the occasional albino

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
031838-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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