Strain Name    : 129S6/SvEv(B6)-Map3k4tm1Glj/Mmnc

Stock Number : 032019-UNC

Gene Information

(provided by MGI)
Target: Map3k4tm1Glj
Name: targeted mutation 1, Gary L Johnson
Chromosome: 17
Alteration at locus: Knock-In
Target: Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Chromosome: 17
Alteration at locus: Knock-In
Genetic Alterations:
Two mutations were generated in exon 21. A silent mutation introduced a SacI site in codon 1357, from CTG to CTC. In codon 1361, a single nucleotide change from AAG to AGG introduced a substitution of lysine for agrginine. The neomycin gene was introduced into intron 21 in the reverse direction.

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line: TC1/TC-1 derived from 129S6/SvEvTac

    Phenotype

    Homozygous: Embryonic and perinatal lethality due to neural tube, skeletal, and placental defects.

    Heterozygous: Similar defects as homozygotes but with reduced penetrance.

    Mammalian Phenotype Terms:
    Allelic Composition: Map3k4tm1Glj/Map3k4tm1Glj (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

    Allelic Composition: Map3k4tm1Glj/Map3k4+ (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

    Strain of Origin: 129S6/SvEvTac

    Strain genetic background: 129S6/SvEvTac

    Strain Development: Original mutant mouse was backcrossed seven times with 129 SVEV. Mice are maintained by heterozygote intercrosses due to lethalitiy of homozygote mutant.

    Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC StrainReference StrainsGenomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    129S6/SvEv(B6)-Map3k4tm1Glj/Mmnc MMRRC:032019-UNC 129S6 C57BL/6J 2521 26 35 0


    Suggested Control Mice:

    Wild-type littermates or 129S6/SvEvTac mice

    • Apoptosis
    • Cell Biology
    • Developmental Biology
    • Immunology and Inflammation
    • Models for Human Disease
    • Reproduction

    Donor: Gary L Johnson, Ph.D., University of North Carolina at Chapel Hill School of Medicine.

    Primary Reference:

    • Abell AN; Rivera-Perez JA; Cuevas BD; Uhlik MT; Sather S; Johnson NL; Minton SK; Lauder JM; Winter-Vann AM; Nakamura K; Magnuson T; Vaillancourt RR; Heasley LE; Johnson GL, Ablation of MEKK4 kinase activity causes neurulation and skeletal patterning defects in the mouse embryo., Mol Cell Biol 2005 Oct;25(20):8948-59 (Medline PMID: 16199873)
    • Abell, A.N., Granger, D.A., Johnson, N.L., Vincent-Jordan, N., Dibble, C.F.and Johnson, G.L. Trophoblast stem cell maintenance by fibroblast growth factor 4 requires MEKK4 activation of Jun N-terminal kinase. Mol Cell Biol 2009 29: 2748-2761 (Medline PMID: 19289495)

    Colony and Husbandry Information

    The mice have an increased frequency of eye infections; this can be treated with antibiotic ointment.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Coat color: Brown

    Other:

    Curly/kinky tail in surviving homozygotes. Increased frequency of eye infections. Small litter size.

    MMRRC Breeding System: Sib-mating

    Breeding Scheme(s):

    • Heterozygous female x Heterozyguos male littermate.
    • Due to placental and developmental defects, a larger breeding colony needed to maintain the colony.

    Generation: N7

    Overall Breeding Performance: Poor: Due to shortened reproductive lifespan, future breeding pairs should be saved from the first litter should a second litter is not produced.

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: No No
    Homozygotes are fertile:
    Heterozygotes are fertile: Reduced Reduced
    Age Reproductive Decline: 5 months 5 months

    Average litter size: 2-3

    Recommended wean age: 3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    032019-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.