Name: targeted mutation 1, Gary L Johnson
Alteration at locus: Knock-In
Name: mitogen-activated protein kinase kinase kinase 4
Alteration at locus: Knock-In
ES Cell Line: TC1/TC-1 derived from 129S6/SvEvTac
Homozygous: Embryonic and perinatal lethality due to neural tube, skeletal, and placental defects.
Heterozygous: Similar defects as homozygotes but with reduced penetrance.
Strain of Origin: 129S6/SvEvTac
Strain genetic background: 129S6/SvEvTac
Strain Development: Original mutant mouse was backcrossed seven times with 129 SVEV. Mice are maintained by heterozygote intercrosses due to lethalitiy of homozygote mutant.
Strain Genetic Background Validation:
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
|MMRRC Strain||Reference Strains||Genomic makeup (Mb)|
|Strain Name||MMRRC ID #||Reference 1||Reference 2||Ref 1||Ref 2||Het||Unknown|
Suggested Control Mice:
Wild-type littermates or 129S6/SvEvTac mice
Donor: Gary L Johnson, Ph.D., University of North Carolina at Chapel Hill School of Medicine.
The mice have an increased frequency of eye infections; this can be treated with antibiotic ointment.
Coat color: Brown
Curly/kinky tail in surviving homozygotes. Increased frequency of eye infections. Small litter size.
MMRRC Breeding System: Sib-mating
Overall Breeding Performance: Poor: Due to shortened reproductive lifespan, future breeding pairs should be saved from the first litter should a second litter is not produced.
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||No||No|
|Homozygotes are fertile:|
|Heterozygotes are fertile:||Reduced||Reduced|
|Age Reproductive Decline:||5 months||5 months|
Average litter size: 2-3
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.