Strain Name:
CB6-Tg(Rbp3-SV40)IT-2Jjw/Mmjax
Stock Number:
032030-JAX
Citation ID:
RRID:MMRRC_032030-JAX

Gene Information

SV40gp6
Name: Simian vacuolating virus 40
Type: Gene
Species: Virus
Rbp3
Name: retinol binding protein 3, interstitial
Synonyms: Irbp, Rbp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19661
VEGA: 14
HGNC: HGNC:9921
Homologene: 9261
Tg(Rbp3-SV40)IT-2Jjw
Name: transgene insertion IT-2, Jolene J Windle
Synonyms: IRBP-Tag
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genotype Determination
Phenotype
Homozygous: Undetermined.

Hemizygous: Mice hemizygous for the IRBP-Tag allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of Tag is regulated by a murine interstitial retinol binding protein (IRBP) promoter (rbp3), which leads to expression of Tag in photoreceptor cells of the developing retina before their terminal differentiation. This expression results in hereditary retinoblastomas and pineal tumors arising from photoreceptor cells and pinealocytes, which may share a common evolutionary ancestor with retinal cells. IRBP-Tag mice develop narrowed ocular fissures by 3 to 4 weeks of age, and exophthalmia by 8 weeks of age. The pineal gland is enlarged by 5 to 6 weeks of age, and is no longer intact by 8 to 10 weeks of age when the tumor covers and invades the cerebral and cerebellar hemispheres. These mice may be useful for studying heritable ocular tumors of photoreceptor cell origin and pineal tumors associated with retinoblastoma.
MeSH Terms
  • Animals
  • Antigens, Polyomavirus Transforming/genetics
  • Antigens, Polyomavirus Transforming/metabolism
  • Blotting, Northern
  • Brain Neoplasms/metabolism
  • Brain Neoplasms/pathology
  • Disease Models, Animal
  • Eye Neoplasms/genetics
  • Eye Neoplasms/metabolism
  • Eye Neoplasms/pathology
  • Eye Proteins
  • Female
  • Gene Expression
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Photoreceptor Cells/metabolism
  • Photoreceptor Cells/pathology
  • Pineal Gland/metabolism
  • Pineal Gland/pathology
  • Retinoblastoma/genetics
  • Retinoblastoma/metabolism
  • Retinoblastoma/pathology
  • Retinol-Binding Proteins/genetics
  • Retinol-Binding Proteins/metabolism
  • Tumor Cells, Cultured
  • Brain/pathology
  • Brain Neoplasms/genetics
  • Crystallins/genetics
  • Eye Proteins/genetics
  • Gene Expression Regulation, Neoplastic
  • Retina/pathology
Strain Development
The IRBP-Tag transgene contains the simian virus 40 (SV40) early region encoding both large T-antigen (Tag) and small t-antigen under the control of murine interstitial retinol binding protein (IRBP) promoter (rbp3). The construct was microinjected into fertilized oocytes obtained from matings of CB6F1/Hsd (BALB/cAnNHsd inbred female bred to C57BL/6NHsd inbred male), and offspring from founder line 2 were bred to CB6F1/Hsd nontransgenic mice to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to CB6F1/J hybrid mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates
Donor
Daniel M. Albert, Ph.D., University of Wisconsin Madison
Primary Reference
  • Howes KA; Lasudry JG; Albert DM; Windle JJ, Photoreceptor cell tumors in transgenic mice., Invest Ophthalmol Vis Sci 1994 Feb;35(2):342-51 (Medline PMID: 8112979)
  • Marcus DM, Lasudry JG, Carpenter JL, Windle J, Howes KA, al-Ubaidi MR, Baehr W, Overbeek PA, Font RL, Albert DM. Trilateral tumors in four different lines of transgenic mice expressing SV40 T-antigen. Invest Ophthalmol Vis Sci. 1996 Feb;37(2):392-6. (Medline PMID: 8603844)

Colony and Husbandry Information

When maintaining a live colony, hemizygous mice may be bred to CB6F1/J hybrid mice. The donating investigator reports always breeding hemizygous mice to CB6F1 mice.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032030-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032030-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.