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Strain Name:
CB6-Tg(TagRb)1Plm/Mmjax
Stock Number:
032031-JAX
Citation ID:
RRID:MMRRC_032031-JAX

Strain Information

Tg(TagRb)1Plm
Name: transgene insertion 1, Pamela L Mellon
Synonyms: hLHB-Tag, LHBETATAG, LHbeta-Tag, LHB-Tag, hLHBeta-Tag, TAg-RB
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Phenotype
Homozygous: Not characterized.

Hemizygous: Mice hemizygous for the LHbeta-Tag allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Although the Tag gene was inserted downstream of the pituitary-specific human luteinizing hormone beta-subunit (LHbeta) promoter, the major sites of Tag expression in this line of mice are the retina and pineal gland. No pituitary phenotype is observed in these mice. This expression results in pineal tumors and bilateral ocular neoplasms with marked resemblance to human retinoblastoma. LHbeta-Tag mice develop retinal tumors by 2 months of age, with the tumor filling the vitreous cavity by 5 months of age. These mice may be useful for studying the process of malignant transformation in retinoblastoma in vivo from inception through extension and metastases, and they provide a stable model for human preclinical studies.
MeSH Terms
  • Animals
  • Antigens, Polyomavirus Transforming/genetics
  • Chromosome Banding
  • Chromosome Mapping
  • Eye Neoplasms/genetics
  • Eye Neoplasms/pathology
  • Eye Neoplasms/ultrastructure
  • Female
  • Gene Expression
  • Humans
  • Luteinizing Hormone/genetics
  • Lymphocytes/cytology
  • Male
  • Mice
  • Mice, Transgenic
  • Nucleic Acid Hybridization
  • RNA/genetics
  • RNA/isolation & purification
  • RNA, Messenger/genetics
  • Retinoblastoma/genetics
  • Retinoblastoma/pathology
  • Retinoblastoma/ultrastructure
  • Simian virus 40/genetics
  • Simian virus 40/immunology
  • Blotting, Southern
  • Brain Neoplasms/genetics
  • Brain Neoplasms/pathology
  • Disease Models, Animal
  • Polymorphism, Restriction Fragment Length
  • Transfection/genetics
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Daniel M. Albert, Ph.D., University of Wisconsin Madison
Primary Reference
  • Windle JJ; Albert DM; O'Brien JM; Marcus DM; Disteche CM; Bernards R; Mellon PL, Retinoblastoma in transgenic mice., Nature 1990 Feb 15;343(6259):665-9 (Medline PMID: 1689463)
  • O'Brien JM, Marcus DM, Niffenegger AS, Bernards R, Carpenter JL, Windle JJ, Mellon P, Albert DM. Trilateral retinoblastoma in transgenic mice. Trans Am Ophthalmol Soc. 1989;87:301-22; discussion 322-6. (Medline PMID: 2576479)
Strain Development
The LHbeta-Tag transgene contains the simian virus 40 (SV40) early region encoding both large T-antigen (Tag) and small t-antigen downstream of the human luteinizing hormone beta-subunit gene (LHbeta) promoter. The construct was microinjected into fertilized oocytes obtained from matings of CB6F1/J (BALB/cJ inbred female and the C57BL/6J inbred male), and offspring from founder line 1 were bred to CB6F1/J nontransgenic mice to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to CB6F1/J hybrid mice or for at least one generation to establish the colony.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

When maintaining a live colony, hemizygous mice may be bred to CB6F1/J hybrid mice. The donating investigator reports always breeding hemizygous mice to CB6F1 mice.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032031-JAX-SPERM Cryo-preserved spermatozoa $459.00 / Non-Profit Aliquot Approximate quantity3
032031-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.