Strain Name:
B6.Cg-Col1a1tm1(CAG-Sirt1)Dsin/Mmjax
Stock Number:
032033-JAX
Citation ID:
RRID:MMRRC_032033-JAX

Strain Information

Col1a1tm1(CAG-Sirt1)Dsin
Name: collagen, type I, alpha 1; targeted mutation 1, David Sinclair
Synonyms: ColA1flox-STOP-Sirt1, SIRT1STOP, Col1a1Sirt1-Tg flox-STOP-flox
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Targeted Mutation
Col1a1
Name: collagen, type I, alpha 1
Synonyms: Col1a-1, Cola-1, Mov-13, Cola1
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Targeted Mutation
NCBI: 12842
HGNC: HGNC:2197
Homologene: 73874
ES Cell Line
Not specified
Phenotype
Mice homozygous for this SIRT1STOP (or SIRT1-Tg flox-STOP-flox) targeted allele are viable and fertile, with a floxed-STOP cassette and mouse sirtuin 1 (Sirt1) cDNA sequence inserted into the 3' UTR of the collagen A1 (Col1a1) locus. When bred to mice that express Cre recombinase, offspring will have the floxed-STOP cassette deleted in the cre-expressing tissue(s), resulting in Sirt1 overexpression in Col1a1-expressing cells. These SIRT1STOP (or SIRT1-Tg flox-STOP-flox) mice may be useful for generating conditional mutations for studying the role of sirtuin NAD+-deacetylases in aging, cancer, cellular stress resistance, genomic stability, IGF1 pathways, autoimmunity, inflammation, T cell tolerance, energy metabolism, neurodegeneration, insulin resistance, and diabetes.

Heterozygous: Normal
MeSH Terms
  • Animals
  • Cell Nucleus/metabolism
  • Cell Proliferation
  • Colonic Neoplasms/enzymology
  • Colonic Neoplasms/pathology
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Models, Biological
  • Rats
  • Rats, Inbred F344
  • Sirtuin 1
  • Sirtuins/metabolism
  • Sirtuins/physiology
  • beta Catenin/metabolism
Strain Development
The SIRT1-Tg flox-STOP-flox construct was designed to insert (from 5' to 3') an FRT site, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a loxP-flanked transcriptional STOP cassette, the mouse sirtuin 1 (Sirt1) cDNA sequence, and a PGK-hygro-polyA sequence (with a second FRT site between the PGK and hygro) into the 3' UTR of the mouse collagen A1 (Col1a1) locus. The targeting vector was electroporated into (C57BL/6 x 129S4Sv/Jae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to generate the colony. These SIRT1STOP mice were backcrossed to C57BL/6J mice for 12 generations prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J inbred mice to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Research Tool
Donor
David Sinclair, Ph.D., Harvard Medical School
Primary Reference
Firestein R; Blander G; Michan S; Oberdoerffer P; Ogino S; Campbell J; Bhimavarapu A; Luikenhuis S; de Cabo R; Fuchs C; Hahn WC; Guarente LP; Sinclair DA, The SIRT1 deacetylase suppresses intestinal tumorigenesis and colon cancer growth., PLoS ONE 2008;3(4):e2020 (Medline PMID: 18414679)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032033-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
032033-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.