Strain Name:
STOCK Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh/Mmjax
Stock Number:
032037-JAX
Citation ID:
RRID:MMRRC_032037-JAX

Gene Information

Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Gordon Fishell
Synonyms: RCE:loxP, RCEfl, RCEfsEGFP, RCE, RCE-LoxP, RCE-GFP, RCEEGFP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Targeted Mutation
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrosa26, Gtrgeo26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
NCBI: 14910
Genotype Determination
ES Cell Line
W4 derived from 129S6/SvEvTac
Phenotype
Homozygous: Mice homozygous for the R26R CAG-boosted EGFP (RCE) reporter allele harboring a loxP-flanked STOP cassette (RCE:loxP) are viable and fertile. Under control of the endogenous Gt(ROSA)26Sor promoter/enhancer regions and the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin (CAG) hybrid promoter, widespread expression of enhanced green fluorescent protein (EGFP) is prevented by the upstream floxed-STOP cassette. After removal of the loxP-flanked STOP cassette by cre-mediated recombination, EGFP reporter expression is directed to the cells/tissues dependent upon expression of the promoter expression driving Cre recombinase.

Heterozygous: Undetermined.
Mammalian Phenotype Terms
Allelic Composition: Gt(ROSA)26Sortm1.1(CAG-EGFP)Fsh/Gt(ROSA)26Sor+ (Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * Swiss Webster )

MeSH Terms
  • Animals
  • Homeodomain Proteins/metabolism
  • Interneurons/cytology
  • Interneurons/physiology
  • Mice
  • Mice, Knockout
  • Neocortex/cytology
  • Neocortex/growth & development
  • Tissue Distribution
  • Transcription Factors/metabolism
Strain Development
The R26R CAG-boosted EGFP (RCE) reporter targeting vector was designed with (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a splice acceptor site, a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA and 3x-polyA), an FRT-flanked modified pBS302 STOP cassette, an enhanced green fluorescent protein (EGFP) sequence, and two AttP-polyA sequences. This entire construct was inserted into the first exon of the Gt(ROSA)26Sor locus via electroporation into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric mice were bred with C57BL/6 mice to generate the RCE:dual colony (see MMRRC:032036). RCE:dual mice were then bred with a germline FLP-expressing strain (unspecified genetic background) to delete the FRT-flanked modified pBS302 STOP cassette. The resulting RCE:loxP offspring were mated with outbred Swiss Webster mice for many generations (selectively removing the FLP-expressing mutation), and subsequently maintained as homozygotes prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were bred to C57BL/6J inbred mice (Jax Stock No. 000664) for at least one generation to establish the RCE:loxP colony.
Suggested Control Mice
Wild-type littermates
Donor
Gord Fishell, Ph.D., New York University School of Medicine
Primary Reference
Sousa VH; Miyoshi G; Hjerling-Leffler J; Karayannis T; Fishell G, Characterization of Nkx6-2-derived neocortical interneuron lineages., Cereb Cortex 2009 Jul;19 Suppl 1:i1-10. (Medline PMID: 19363146)

Colony and Husbandry Information

When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. Homozygous mice may also be bred together.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032037-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032037-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.