Strain Name:
B6.129X1(FVB)-Cd28tm2.1Jmg/Mmjax
Stock Number:
032040-JAX
Citation ID:
RRID:MMRRC_032040-JAX

Strain Information

Cd28tm2.1Jmg
Name: CD28 antigen; targeted mutation 2.1, Jonathan M Green
Synonyms: CD28-Y170F
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knock-In
Cd28
Name: CD28 antigen
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knock-In
NCBI: 12487
HGNC: HGNC:1653
Homologene: 4473
Genotype Determination
ES Cell Line
RW-4 derived from 129X1/SvJ
Phenotype
Homozygous: Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice carry a mutant allele with a phenylalanine substitution for the tyrosine at position 170 in exon 4 (CD2- Y170F), which encodes proximal tyrosine-based binding motif. The substitution was confirmed by direct sequencing and RT-PCR. Mutant protein expression levels on thymocytes and peripheral T cells are similar to wildtype protein levels in control mice, as assayed by flow cytometry. Homozygotes have IL-2 production and secretion similar to wildtype controls. This mutant mouse strain may be useful in studies of humoral immune response and T cell proliferation.

Heterozygous: Undetermined.
Mammalian Phenotype Terms
Allelic Composition: Cd28tm2.1Jmg/Cd28tm2.1Jmg (Genetic Background: B6.129X1-Cd28tm2.1Jmg )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Antibody Formation
  • Bronchial Hyperreactivity/immunology
  • CD28 Antigens/chemistry
  • CD28 Antigens/genetics
  • CD28 Antigens/immunology
  • CD28 Antigens/metabolism
  • Cell Communication
  • Cell Proliferation
  • Dose-Response Relationship, Immunologic
  • Germinal Center/cytology
  • Germinal Center/immunology
  • Immunoglobulin G/metabolism
  • Interleukin-2/metabolism
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Proline/chemistry
  • Proline/metabolism
  • Signal Transduction
  • T-Lymphocytes/immunology
  • bcl-X Protein/metabolism
  • Amino Acid Motifs
  • Encephalomyelitis, Autoimmune, Experimental/immunology
  • Enzyme Activation
  • Inflammation/immunology
  • Interleukin-2/genetics
  • Interleukin-4/immunology
  • Interleukin-4/secretion
  • Lymphocyte Activation/immunology
  • Mice, Inbred BALB C
  • Mice, Transgenic
  • Phosphatidylinositol 3-Kinases/genetics
  • Phosphatidylinositol 3-Kinases/metabolism
  • Proto-Oncogene Proteins c-akt/genetics
  • Proto-Oncogene Proteins c-akt/metabolism
  • Signal Transduction/physiology
  • Spleen/cytology
  • T-Lymphocytes/metabolism
  • Thymus Gland/cytology
Strain Development
A targeting vector containing a floxed neo cassette was used to insert a phenylalanine substitution for the tyrosine at position 170 in exon 4. The construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to a transgenic EllA-cre strain on the C57BL/6 background to remove the floxed neo selection cassette. The mice were then backcrossed to C57BL/6 for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J once to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
Donor
Jonathan Green, Ph.D., Washington University School of Medicine
Primary Reference
  • Friend LD; Shah DD; Deppong C; Lin J; Bricker TL; Juehne TI; Rose CM; Green JM, A dose-dependent requirement for the proline motif of CD28 in cellular and humoral immunity revealed by a targeted knockin mutant., J Exp Med 2006 Sep 4;203(9):2121-33 (Medline PMID: 16908623)
  • Dodson LF; Boomer JS; Deppong CM; Shah DD; Sim J; Bricker TL; Russell JH; Green JM, Targeted knock-in mice expressing mutations of CD28 reveal an essential pathway for costimulation., Mol Cell Biol 2009 Jul;29(13):3710-21 (Medline PMID: 19398586)

Colony and Husbandry Information

When maintaining a live colony, these mice can be bred as homozygotes.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032040-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032040-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.