Strain Name:
C.B6-Tg(Foxp3-DTR/EGFP)23.2Spar/Mmjax
Stock Number:
032049-JAX
Citation ID:
RRID:MMRRC_032049-JAX

Gene Information

EGFP
Name: enhanced green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Foxp3
Name: forkhead box P3
Synonyms: scurfin, JM2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20371
HGNC: HGNC:6106
Homologene: 8516
DTR
Name: diphtheria toxin receptor
Type: Gene
Species: Virus
Tg(Foxp3-DTR/EGFP)23.2Spar
Name: transgene insertion 23.2, Tim Sparwasser
Synonyms: Tg(Foxp3-DTR/EGFP)1Ebe, DEREG
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (BAC)
Genetic Alterations
The RP23-267C15 bacterial artificial chromosome (BAC) containing at least ten mouse genes was obtained. This BAC was modified by targeting a simian diphtheria toxin receptor-enhanced green fluorescent protein (DTR-eGFP) fusion protein and an SV40 polyA element into the first exon of the Foxp3 (forkhead box P3) locus: none of the other loci on the BAC were mutated.
Phenotype
Homozygous: The donating investigator reports that while homozygous females breed fine, homozygous males seem infertile.

Hemizygous: Mice hemizygous for the "depletion of regulatory T cell" (DEREG) BAC transgene are viable and fertile, with expression of a simian diphtheria toxin receptor-enhanced green fluorescent protein (DTR-eGFP) fusion protein under control of the endogenous Foxp3 (forkhead box P3) promoter/enhancer regions on the BAC transgene. The donating investigator reports that transcription/translation from the BAC Foxp3 locus is disabled. These DEREG mice have DTR-eGFP expression in fully functional Foxp3+CD4+ regulatory T cell populations with no observed influence on regulation of the endogenous Foxp3 locus. Specifically, EGFP expression (via FACS) is observed mainly in CD25+CD4+ T cells, and allows specific detection of Foxp3+ regulatory T cell populations. The donating investigator also reports that EGFP expression is measurable by direct fluorescence. Diphtheria toxin (DT) administration results in ablation of Foxp3+CD4+ T reg cells with no apparent affect on CD25+ effector T cells. DT-induced depletion of Foxp3+CD4+ T reg cells is associated with enhanced and prolonged delayed-type hypersensitivity (DTH) responses and neonatal development of scurfy-like symptoms.
MeSH Terms
  • Animals
  • Animals, Newborn
  • Base Sequence
  • Chromosomes, Artificial, Bacterial/genetics
  • DNA Primers/genetics
  • Diphtheria Toxin/pharmacology
  • Forkhead Transcription Factors/deficiency
  • Forkhead Transcription Factors/genetics
  • Green Fluorescent Proteins/genetics
  • Heparin-binding EGF-like Growth Factor
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Lymphoproliferative Disorders/etiology
  • Lymphoproliferative Disorders/genetics
  • Lymphoproliferative Disorders/immunology
  • Lymphoproliferative Disorders/pathology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Phenotype
  • Receptors, Cell Surface/genetics
  • Recombinant Fusion Proteins/genetics
  • T-Lymphocytes, Regulatory/immunology
Strain Development
The modified BAC transgene was microinjected into the pronuclei of fertilized C57BL/6NCrl oocytes. Transgenic mice were identified and bred to C57BL/6J wild-type mice to establish the high transgene expressing founder line 23.2. These "depletion of regulatory T cell" (DEREG) mice were maintained on a C57BL/6J genetic background. Next, transgenic mice were backcrossed to BALB/cJ wild-type mice for 10 generations (five "speed-congenic" backcrosses and then five "conventional" backcrosses). After this, transgenic mice were backcrossed 2-3 generations to BALB/cByJ wild-type mice prior to arrival at MMRRC. Upon arrival, mice were bred to BALB/cByJ inbred mice for at least one generation to establish the colony.

The allele in this mutant line is on a genetic background different from that on which the allele was first characterized.

Suggested Control Mice
Wild-type littermates
Donor
Tim Sparwasser, Ph.D., Twincore, Zentrum fur Esperimentelle und Klin. Infektionsforschund GmbH
Primary Reference
Lahl K; Loddenkemper C; Drouin C; Freyer J; Arnason J; Eberl G; Hamann A; Wagner H; Huehn J; Sparwasser T, Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease., J Exp Med 2007 Jan 22;204(1):57-63 (Medline PMID: 17200412)

Colony and Husbandry Information

When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to BALB/cByJ inbred mice. The donating investigator reports that while homozygous females breed fine, homozygous males seem infertile.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032049-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032049-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.