Strain Name    : C57BL/6-Tg(Foxp3-DTR/EGFP)23.2Spar/Mmjax

Stock Number : 032050-JAX

Gene Information

(provided by MGI)
Transgene: Tg(Foxp3-DTR/EGFP)23.2Spar
Name: transgene insertion 23.2, Tim Sparwasser
Alteration at locus: Transgenic
Reporter: EGFP (Jelly Fish)
Name: Enhanced Green Fluorescent Protein
Alteration at locus: Transgenic
Promoter: Foxp3
Name: forkhead box P3
Alteration at locus: Transgenic
Transgene: DTR (Simian)
Name: diphtheria toxin receptor
Alteration at locus: Transgenic
Genetic Alterations:
The RP23-267C15 bacterial artificial chromosome (BAC) containing at least ten mouse genes was obtained. This BAC was modified by targeting a simian diphtheria toxin receptor-enhanced green fluorescent protein (DTR-eGFP) fusion protein and an SV40 polyA element into the first exon of the Foxp3 (forkhead box P3) locus: none of the other loci on the BAC were mutated.

Genotype Determination:

ES Cell Line: Not applicable

Phenotype

Homozygous: The donating investigator reports that while homozygous females breed fine, homozygous males seem infertile.

Hemizygous: Mice hemizygous for the "depletion of regulatory T cell" (DEREG) BAC transgene are viable and fertile, with expression of a simian diphtheria toxin receptor-enhanced green fluorescent protein (DTR-eGFP) fusion protein under control of the endogenous Foxp3 (forkhead box P3) promoter/enhancer regions on the BAC transgene. The donating investigator reports that transcription/translation from the BAC Foxp3 locus is disabled. These DEREG mice have DTR-eGFP expression in fully functional Foxp3+Foxp3 locus. Specifically, EGFP expression (via FACS) is observed mainly in CD25+CD4+ T cells, and allows specific detection of Foxp3+ regulatory T cell populations. The donating investigator also reports that EGFP expression is measurable by direct fluorescence. Diphtheria toxin (DT) administration results in ablation of Foxp3+CD4+ T reg cells with no apparent affect on CD25+ effector T cells. DT-induced depletion of Foxp3+CD4+ T reg cells is associated with enhanced and prolonged delayed-type hypersensitivity (DTH) responses and neonatal development of scurfy-like symptoms.

Mammalian Phenotype Terms:
Allelic Composition: Tg(Foxp3-DTR/EGFP)23.2Spar/0 (Genetic Background: involves: C57BL/6NCrl )

Strain of Origin: C57BL/6N

Strain genetic background: C57BL/6

Strain Development: This modified BAC transgene was microinjected into the pronuclei of fertilized C57BL/6NCrl oocytes. Transgenic mice were identified and bred to C57BL/6J wild-type mice to establish the high transgene expressing founder line 23.2. These "depletion of regulatory T cell" (DEREG) mice were subsequently maintained by breeding transgenic mice with wild-type C57BL/6J or wild-type siblings for many generations prior to arrival at MMRRC. Upon arrival, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.

The allele in this mutant line is on a genetic background different from that on which the allele was first characterized.

Suggested Control Mice: Wild-type littermates

Donor: Tim Sparwasser, Ph.D., Twincore, Zentrum fur Esperimentelle und Klin. Infektionsforschund GmbH

Primary Reference:

Lahl K; Loddenkemper C; Drouin C; Freyer J; Arnason J; Eberl G; Hamann A; Wagner H; Huehn J; Sparwasser T, Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease., J Exp Med 2007 Jan 22;204(1):57-63 (Medline PMID: 17200412)

Colony and Husbandry Information

When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) siblings or to C57BL/6J inbred mice. The donating investigator reports that while homozygous females breed fine, homozygous males seem infertile.

Colony's Current Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
032050-JAX-HEMI-FHemizygous female
032050-JAX-HEMI-MHemizygous male
032050-JAX-WT-FWild type female
032050-JAX-WT-MWild type male
$218.00
Non-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.