Loading...

Strain Detail Sheet

Strain Name:
C57BL/6-Miftm2Gfr/Mmjax
Stock Number:
032057-JAX
Citation ID:
RRID:MMRRC_032057-JAX

Strain Information

Gene Details

Miftm2Gfr
Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor); targeted mutation 2, G Fingerle-Rowson
Synonyms: mifP1G
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knock-In
Mif
Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Synonyms: Glif
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knock-In
NCBI: 17319
VEGA: 10
HGNC: HGNC:7097
Homologene: 55655

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genotype Determination
ES Cell Line
Bruce4 derived from B6.PL-Thy1a/CyJ

Strain Description

Phenotype
Homozygous: The MifP1G allele replaces the endogenous Mif gene with a mutant form of MIF that lacks the tautomerase activity (P1G-MIF). Homozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice have some reduction in binding to cell surface receptors compared to wildtype mice. As Mif is a widely expressed cytokine and upstream regulator of the immune response by inhibiting the proapoptotic and cell cycle-regulatory function of the p53 tumor suppressor, these MifP1G mice may be useful for studying the structure and function of MIF, innate- and autoimmune disorders, inflammation and cancer, and tumorigenesis caused by neoangiogenesis.

Heterozygous: Undetermined.
Mammalian Phenotype Terms
Allelic Composition: Miftm2Gfr/Miftm2Gfr (Genetic Background: C57BL/6-Miftm2Gfr )
Strain Development
The targeting vector was designed to create a proline to glycine mutation (Pro1Gly or P1G) in exon 1 of Macrophage migration-inhibitory factor (Mif), and to insert a self-excising loxP-flanked cassette (itself containing a neomycin resistance (Neo) gene and a testes-specific angiotensin-converting enzyme promoter-driven Cre recombinase gene (tACE-Cre)). The construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts and resulting chimeric males were bred to C57BL/6Crl females. Mice heterozygous for this MifP1G allele were bred together to generate homozygotes. Upon arrival mice at The Jackson Laboratory these mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Strain Origin

Donor
Richard Bucala, Ph.D., Yale School of Medicine
Primary Reference
Fingerle-Rowson G, Kaleswarapu DR, Schlander C, Kabgani N, Brocks T, Reinart N, Busch R, Sch

Colony and Husbandry Information

Special Considerations

When maintaining a live colony, homozygous mice may be bred together.

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
032057-JAX-SPERM Cryo-preserved spermatozoa $459.00 / $459.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
032057-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / $2,123.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.