Strain Name:
B6;129X1-Park7tm1Cai/Mmjax
Stock Number:
032090-JAX
Citation ID:
RRID:MMRRC_032090-JAX

Gene Information

Park7tm1Cai
Name: Parkinson disease (autosomal recessive, early onset) 7; targeted mutation 1, Hubaibin Cai
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Park7
Name: Parkinson disease (autosomal recessive, early onset) 7
Synonyms: DJ-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
NCBI: 57320
Homologene: 38295
Genetic Alterations
Targeted deletion of mouse DJ-1 was accomplished by replacing exon 2 with a neomycin resistance gene cassette.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous phenotype: Mice that are homozygous for the targeted mutation exhibit non-progressive hypokinesia at 5 months of age and progressive gait abnormalities (stride uniformity, hind base displacement) beginning at two months of age. Mice over a year old develop decreased grip strength, loss of body weight and severe gait bnormalities. Although early onset progressive motor deficits occur in these mice, no pathological changes are observed in the nigrostriatal system, spinal motor system or muscles. This mutant mouse strain may be useful in studies of Parkinson's disease.

Heterozygous phenotype: DJ- 1+/- mice show no obvious phenotypes compared to wild-type littermates.
Mammalian Phenotype Terms
Allelic Composition: Park7tm1Cai/Park7tm1Cai (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

MeSH Terms
  • Animals
  • Behavior, Animal/physiology
  • Corpus Striatum/physiology
  • Disease Progression
  • Maze Learning/physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microtubule-Associated Proteins/deficiency
  • Microtubule-Associated Proteins/genetics
  • Motor Activity/physiology
  • Substantia Nigra/physiology
Strain Development
A targeting vector containing a neomycin resistance cassette was used to disrupt exon 2. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and maintained by intercross. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Cell Biology
  • Models for Human Disease
  • Neurobiology
Donor
Huaibin Cai, Ph.D., NIH
Primary Reference

Chandran JS, Lin X, Zapata A, Höke A, Shimoji M, Moore SO, Galloway MP, Laird FM, Wong PC, Price DL, Bailey KR, Crawley JN, Shippenberg T, Cai H. Progressive behavioral deficits in DJ-1-deficient mice are associated with normal nigrostriatal function. Neurobiol Dis. 2008 Mar;29(3):505-14. Epub 2007 Dec 4. (Medline PMID: 18187333)

While maintaining a live colony, these mice are bred as homozygotes
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032090-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032090-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.