Strain Name:
B6.129X1-Slc17a7tm1Edw/Mmucd
Stock Number:
032097-UCD
Citation ID:
RRID:MMRRC_032097-UCD
Other Names:
VGLUT1 KO, B6.129X1-Slc17a7tm1Edw/Mmcd

Strain Information

Slc17a7tm1Edw
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; targeted mutation 1, Robert H Edwards
Synonyms: VGLUT1-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Slc17a7
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Synonyms: Vglut1, 2900052E22Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 72961
Homologene: 113454
Genetic Alterations
Neo cassette replaces exon 2 and 3.
Genotype Determination
ES Cell Line
JM-1 derived from 129X1/SvJ
Phenotype
Homozygous: blind, high mortality rate at weaning age, loss of excitatory neurotransmission in hippocampus.

Heterozygous: no observed phenotype
Mammalian Phenotype Terms
Allelic Composition: Slc17a7tm1Edw/Slc17a7tm1Edw (Genetic Background: involves: 129X1/SvJ )

MeSH Terms
  • Animals
  • Animals, Newborn
  • Brain/cytology
  • Brain/metabolism
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Cell Membrane/physiology
  • Cells, Cultured
  • Cerebellum/metabolism
  • Cerebellum/ultrastructure
  • Excitatory Postsynaptic Potentials
  • Glutamic Acid/metabolism
  • Hippocampus/cytology
  • Hippocampus/metabolism
  • Hippocampus/ultrastructure
  • In Situ Hybridization
  • Membrane Transport Proteins
  • Mice
  • Mice, Knockout
  • Nerve Tissue Proteins/metabolism
  • Neurons/metabolism
  • Neurons/physiology
  • Patch-Clamp Techniques
  • Purkinje Cells/physiology
  • Pyramidal Cells/metabolism
  • Synapses/metabolism
  • Synapses/ultrastructure
  • Synaptic Transmission
  • Synaptic Vesicles/metabolism
  • Synaptic Vesicles/physiology
  • Vesicular Glutamate Transport Protein 1
  • Vesicular Glutamate Transport Protein 2
  • Vesicular Transport Proteins
  • 3T3 Cells
  • Bone Resorption/metabolism
  • Cell Line
  • Exocytosis/physiology
  • Homeostasis
  • In Vitro Techniques
  • Mice, Inbred C57BL
  • Microscopy, Immunoelectron
  • Models, Biological
  • Osteoclasts/metabolism
  • Osteoclasts/ultrastructure
  • Receptors, Metabotropic Glutamate/agonists
  • Receptors, Metabotropic Glutamate/antagonists & inhibitors
  • Receptors, Metabotropic Glutamate/metabolism
  • Signal Transduction
  • Vesicular Glutamate Transport Protein 1/deficiency
  • Vesicular Glutamate Transport Protein 1/genetics
Strain Development
Founder was backcrossed with C57BL/6 for 5 generations, and then mated with siblings.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Bone Development
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Osteoporosis
  • Research Tools
Donor
Robert Edwards, M.D., University of California, San Francisco
Primary Reference
  • Fremeau RT Jr, Kam K, Qureshi T, Johnson J, Copenhagen DR, Storm-Mathisen J, Chaudhry FA, Nicoll RA, Edwards RH. Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites. Science. 2004 Jun 18;304(5678):1815-9. Epub 2004 Apr 29.PMID: 15118123 (Medline PMID: 15118123)
  • [Citation)]Johnson J, Fremeau RT Jr, Duncan JL, Renter
  • Morimoto R, Uehara S, Yatsushiro S, Juge N, Hua Z, Senoh S, Echigo N, Hayashi M, Mizoguchi T, Ninomiya T, Udagawa N, Omote H, Yamamoto A, Edwards RH, Moriyama Y. Secretion of L-glutamate from osteoclasts through transcytosis. EMBO J. 2006 Sep 20;25(18):4175-86. Epub 2006 Sep 7. (Medline PMID: 16957773)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032097-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
032097-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
032097-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.